Food Analytical Methods

, Volume 10, Issue 6, pp 2053–2059 | Cite as

Preparation of Multi-target Fusarium Toxins (Zearalenone, Deoxynivalenol, T-2, and HT-2 Toxins) Immunoaffinity Column Using Polystyrene-Divinylbenzene as Matrix

Article
  • 162 Downloads

Abstract

The aim of this study was to establish a purifying immunoaffinity column (IAC) for four kinds of Fusarium toxins (ZEN, DON, T-2, and HT-2 toxins) simultaneously, by using polystyrene-divinylbenzene (PS-DVB) as a column matrix. Four different types of PS-DVB resins were selected to prepare the IACs. Among them, two types of resins, GALAK and Agela, were chosen for IAC preparation. The coupling ratios of the monoclonal antibodies and GALAK or Agela resins were 98.37 and 97.64%, the recoveries of the four Fusarium toxins on the GALAK or Agela IACs were 73.02∼91.97 or 66.16∼112.80%, and the RSDs of recoveries were 2.17∼16.53 or 3.42∼12.25%. They can be applied for the purification of Fusarium toxins from the actual food samples. The GALAK resin was a type of matrix with hydrophilic treatment. The recoveries of four Fusarium toxins on Agela IAC were either too low or too high. The adsorption capacities of the GALAK IAC for the four Fusarium toxins were better than those of the Agela IAC. Hence, the research developed a new method for the preparation of multi-target IAC using PS-DVB resin as matrix of column for determination of Fusarium toxins in food. Then, the GALAK IAC was used for flour samples to detect the feasibility of the application in the actual sample.

Keywords

Polystyrene-divinylbenzene Immunoaffinity columns Fusarium toxins 

Notes

Acknowledgements

The authors would like to express their gratitude to the Beijing Natural Science Fund (14L00184), the Importation and Development of High-Caliber Talents Project of Beijing Municipal Institutions (CIT&TCD20154045), and The Degree and Graduate Education Reform and Development Project of BUA in 2016 (2016YJS051) for the financial support of this study, Beijing University of Agriculture [funding to improve research quality (QKZ2012003)].

Compliance with Ethical Standards

Conflict of Interest

Jianing Zhang declares that she has no conflict of interest. Ke Ding declares that he has no conflict of interest. Tao Han declares that he has no conflict of interest. Xiangning Chen declares that she has no conflict of interest.

Ethical Approval

This article does not contain any studies with human participants or animals performed by any of the authors.

Informed Consent

Not applicable.

References

  1. Arunachalam C, Doohan FM (2013) Trichothecene toxicity in eukaryotes: cellular and molecular mechanisms in plants and animals. Toxicol Lett 217:149–158. doi: 10.1016/j.toxlet.2012.12.003 CrossRefGoogle Scholar
  2. Bennet JW, Klich M (2003) Mycotoxins. Clin Microbiol Rev 16:497–516. doi: 10.1128/CMR.16.3.497-516.2003 CrossRefGoogle Scholar
  3. Booth C (1972) The genus Fusarium. Taxon 21:180. doi: 10.2307/1219251 CrossRefGoogle Scholar
  4. Chen H, Bao C (2016) Study on the disitribution of DON in contaminated wheat. Cereals Oils 29:67–68. doi: 10.3969/j.issn.1008-9578.2016.01.018 Google Scholar
  5. Fujimoto JM, Wang RIH (1970) A method of idemifying narcotic analysis in human urine after theraoeutic doses. Toxicol Appl Pharmacol 16:186–193. doi: 10.1016/0041-008X(70)90175-4 CrossRefGoogle Scholar
  6. Gao S, Deng Y, Zhang C et al (2015) Effects of Fusarium toxin zearalenone (ZEA) and/or deoxynivalenol (DON) on liver function and apoptosis of hepatocyte in mice. Chinese J Vet Sci 35:2021–2026. doi: 10.16303/j.cnki.1005-4545.2015.12.25 Google Scholar
  7. Ge W, Liu X, Zhang W et al (2016) Research progress on detection and detoxification of ZEN in feed. J Anhui Agric Sci 44:95–97. doi: 10.3969/j.issn.0517-6611.2016.01.032 Google Scholar
  8. Ginkel LA, Stephany RW, Rossum HJV (1992) Development and validation of a multiresidue method for beta-agonists in biological samples and animal feed. J AOAC Int 75:554–560Google Scholar
  9. He R, Cao G, Chen M et al (2007) Preparation of polystyrene microspheres with sulfonic acid group on the surface and adsorption of bovine serum albumin. Chem Ind Eng Prog 26:991–994. doi: 10.16085/j.issn.1000-6613.2007.07.015 Google Scholar
  10. Li L, Shang S (2015) Advances in toxicity, mechanism of action and metabolism of T-2 toxin. Hubei Agric Sci 54:5207–5210. doi: 10.14088/j.cnki.issn0439-8114.2015.21.002 Google Scholar
  11. Li Y, Wang Y, Yang H et al (2010) Establishment of an immunoaffinity chromatography for simultaneously selective extraction of Sudan I, II, III and IV from food samples. J Chromatogr A 1217:7840–7847. doi: 10.1016/j.chroma.2010.10.077 CrossRefGoogle Scholar
  12. Liang Y (2014) The study of the methods for the determination of three major Fusarium mycotoxins in formula feed. Dissertation, Northwest A&F UniversityGoogle Scholar
  13. Malik AK, Blasco C, Picó Y (2010) Liquid chromatography-mass spectrometry in food safety. J Chromatogr A 1217:4018–4040. doi: 10.1016/j.chroma.2010.03.015 CrossRefGoogle Scholar
  14. Mei L, Cao B, Yang H et al (2014) Development of an immunoaffinity chromatography column for selective extraction of a new agonist phenylethylamine A from feed, meat and liver samples. J Chromatogr B 945-946:178–184. doi: 10.1016/j.jchromb.2013.11.057 CrossRefGoogle Scholar
  15. Picó Y, Font G, María JR et al (2006) Control of pesticide residues by liquid chromatography-mass spectrometry to ensure food safety. Mass Spectrom Rev 25:917–960. doi: 10.1002/mas.20096 CrossRefGoogle Scholar
  16. Qiu M, Chen H, Sun L et al (2015) Genotoxicity biotransformation products of low-dose T-2/HT-2 toxin. Mod Food Sci Technol 31:42–46. doi: 10.13982/j.mfst.1673-9078.2015.8.008 Google Scholar
  17. Romagnoli B, Ferrari M, Bergamini C (2010) Simultaneous determination of deoxynivalenol, zearalenone, T-2 and HT-2 toxins in breakfast cereals and baby food by high-performance liquid chromatography and tandem mass spectrometry. J Mass Spectrom 45:1075–1080. doi: 10.1002/jms.1802 CrossRefGoogle Scholar
  18. Rotter BA (1996) Invited review: toxicology of deoxynivalenol (vomitoxin). J Toxicol Environ Health 48:1–34. doi: 10.1080/009841096161447 CrossRefGoogle Scholar
  19. Visconti A, Lattanzio VMT, Pascale M et al (2005) Analysis of T-2 and HT-2 toxins in cereal grains by immunoaffinity clean-up and liquid chromatography with fluorescence detection. J Chromatogr A 1075:151–158. doi: 10.1016/j.chroma.2005.04.009 CrossRefGoogle Scholar
  20. Wang Y, Xu Y, Zhang X et al (2011) Development and characterization of a chitosan-supported immunoaffinity chromatography column for the selective extraction of methandrostenolone from food and feed samples. Int J Biol Macromol 49:428–432. doi: 10.1016/j.ijbiomac.2011.05.027 CrossRefGoogle Scholar
  21. Wild CP, Gong YY (2010) Mycotoxins and human disease: a largely ignored global health issue. Carcinogenesis 31:71–82. doi: 10.1093/carcin/bgp264 CrossRefGoogle Scholar
  22. Xin Y, Zhang Y, Wang S et al (2015) Determination of 13 mycotoxins in corn by UPLC-MS/MS. J Chinese Cereals Oils Assoc 30:126–130. doi: 10.3969/j.issn.1003-0174.2015.12.023 Google Scholar
  23. Xu X, Song W, Zhao W (2014) Preparation of polybutadiene grafted polystyrene and epoxy propyl methacrylate copolymer latex and its influence on cement hydration. China Synth Rubber Ind 37:178–181. doi: 10.3969/j.issn.1000-1255.2014.03.005 Google Scholar
  24. Zhang H, Wang M, Wang Y et al (2013) Preparation and application of animmunoaffinity column based on an antibody with strong affinity and packing material with good stability. Food Addit Contam 30:853–860. doi: 10.1080/19440049.2013.789932 CrossRefGoogle Scholar
  25. Zhang J, Liu X, Ding L et al (2014) Synthesis and characterization of tentacular hydrophic boron adsorption hydroxylamine resin. Polym Mater Sci Eng 30:6–10Google Scholar
  26. Zhu Q, Suo L, Hu M et al (2014) Simultaneous determination of 7 mycotoxins in wheat flour by ultra performance liquid chromatography-tandem mass spectrometry using isotope internal standard. Mod Prev Med 42:4362–4377Google Scholar

Copyright information

© Springer Science+Business Media New York 2016

Authors and Affiliations

  • Jianing Zhang
    • 1
  • Ke Ding
    • 1
  • Tao Han
    • 1
  • Xiangning Chen
    • 1
  1. 1.Beijing University of AgricultureBeijingChina

Personalised recommendations