Food Analytical Methods

, Volume 10, Issue 6, pp 2018–2026 | Cite as

High Internal Phase Emulsion Polymeric Monolith Extraction Coupling with High-Performance Liquid Chromatography for the Determination of Para Red and Sudan Dyes in Chilli Samples

  • Fuyou Du
  • Xian Zheng
  • Jianchao Deng
  • Jiao Zou
  • Qiulian Zeng
  • Jianping Li
  • Guihua Ruan


Polymerized high internal phase emulsion (polyHIPE) monoliths were synthesized by in situ polymerization of the continuous phase of a high internal phase emulsion (HIPE) in syringes and successfully applied as monolithic sorbents to extract Para Red and Sudan dyes in chilli samples. Several parameters affecting the extraction efficiency were investigated, including the type of desorption solvent, sample loading rate, sample volume, and pH. Under the optimized conditions, a method using a monolithic polyHIPE column combined with high-performance liquid chromatography (HPLC) was developed for the simultaneous extraction and sensitive determination of Para Red and Sudan dyes in chilli samples. The proposed method had good linearity, with correlation coefficients (R 2) from 0.9985 to 0.9995 and low detection limits (LODs, S/N = 3) in the range 1.5–12 ng/mL for the five analytes. The mean recoveries were ranged from 82.6 to 114.3% with relative standard deviations (RSDs) lower than 4.2%.


High internal phase emulsion Monolithic column Solid-phase extraction Para Red Sudan dyes 



This study was funded by the National Natural Science Foundation of China (Grant Nos. 21265004, 21465008, and 21665006), the Natural Science Foundation from Guangxi Zhuang Autonomous Region (Nos. 2014GXNSFAA118063 and 2015GXNSFAA139024), the Project of High Level Innovation Team and Outstanding Scholar in Guangxi Colleges and Universities (Guijiaoren[2014]49), and the Guangxi Colleges and Universities Key Laboratory of Food Safety and Detection.

Compliance with Ethical Standards

This is an original research article that has not been under editorial review or published or accepted for publication elsewhere. All authors named in the manuscript are entitled to the authorship and have approved the final version of the submitted manuscript.

Conflict of Interest

Fuyou Du declares that he has no competing interests. Xian Zheng declares that he has no competing interests. Jianchao Deng declares that he has no competing interests. Jiao Zou declares that he has no competing interests. Qiulian Zeng declares that he has no competing interests. Jianping Li declares that he has no competing interests. Guihua Ruan declares that he has no competing interests.

Ethical Approval

This article does not contain any studies with human participants or animals performed by any of the authors.

Informed Consent

Informed consent is not applicable for the nature of this study.

Supplementary material

12161_2016_751_MOESM1_ESM.doc (5 mb)
ESM 1 (DOC 5156 kb)


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Copyright information

© Springer Science+Business Media New York 2016

Authors and Affiliations

  1. 1.Guangxi Key Laboratory of Electrochemical and Magnetochemical Functional Materials, College of Chemistry and BioengineeringGuilin University of TechnologyGuilinChina
  2. 2.Key Laboratory of Aquatic Product Processing, Ministry of Agriculture, South China Sea Fisheries Research InstituteChinese Academy of Fishery SciencesGuangzhouChina
  3. 3.Guangxi Collaborative Innovation Center for Water Pollution Control and Water Safety in Karst AreaGuilin University of TechnologyGuilinChina

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