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Food Analytical Methods

, Volume 10, Issue 6, pp 1857–1869 | Cite as

Comparative Analysis of the Volatile Profile of 20 Commercial Samples of Truffles, Truffle Sauces, and Truffle-Flavored Oils by Using HS-SPME-GC-MS

  • Elisabetta Torregiani
  • Sophie Lorier
  • Gianni Sagratini
  • Filippo Maggi
  • Sauro Vittori
  • Giovanni Caprioli
Article

Abstract

The aroma profile of raw truffles, of truffle sauces, and of natural and artificial truffle flavored oils made from or made to imitate Tuber magnatum, Tuber melanosporum, and Tuber aestivum was characterized by solid-phase microextraction-gas chromatography-mass spectrometry (SPME-GC-MS). Both naturally and artificially made oils were not only mainly dominated by bis(methylthio)methane (BMTM), a marker compound typical of white truffle, but also found in most of the oil samples flavored with black truffle. BMTM was not detected or detected in low amounts in black truffles but was very high in sauces (59.74–77.691%); instead, 1-octen-3-ol was high in truffles (35.227–75.208%) but low in sauces. Along the same lines, terpenoid compounds such as α-cubebene, copaene, caryophyllene, α-caryophyllene, and α-farnesene were not detected at all in T. aestivum raw truffle but were present in most truffle sauces. Thus, it was found that neither the natural nor the artificial truffle oil samples adequately replicated the aromas of the species of truffle examined, and this was confirmed by principal component analysis (PCA).

Keywords

Truffle Truffle flavored oils Truffle sauces HS-SPME GC-MS 

Abbreviations

HS

Headspace

SPME

Solid-phase microextraction

GC

Gas chromatography

MS

Mass spectrometry

VOC

Volatile organic compound

Notes

Acknowledgements

The authors would like to thank Sheila Beatty for her editing of the English usage in the manuscript and Urbani Tartufi S.r.l. (Sant’Anatolia di Narco, Perugia, Italy) for economic support.

Compliance with Ethical Standards

Funding

Funding information is not available.

Conflict of Interest

Elisabetta Torregiani declares that she has no conflict of interest. Sophie Lorier declares that she has no conflict of interest. Gianni Sagratini declares that he has no conflict of interest. Filippo Maggi declares that he has no conflict of interest. Sauro Vittori declares that he has no conflict of interest. Giovanni Caprioli declares that he has no conflict of interest.

Ethical Approval

This article does not contain any studies with human participants or animals performed by any of the authors.

Informed Consent

Not applicable.

References

  1. Bellesia F, Pinetti A, Bianchi A, Tirillini B (1996) Volatile compounds of white truffle (T. magnatum Pico) from middle Italy. Flavour Frag J 11:239–243CrossRefGoogle Scholar
  2. Bellesia F, Pinetti A, Bianchi A, Tirillini B (1998) Volatile organic compounds of black truffle (T. melanosporum Vitt.) from middle Italy. Flavour Frag J 13:56–58CrossRefGoogle Scholar
  3. Culleré L, Ferreira V, Chevret B et al (2010) Characterisation of aroma active compounds in black truffles (Tuber melanosporum) and summer truffles (Tuber aestivum) by gas chromatography-olfactometry. Food Chem 122:300–306CrossRefGoogle Scholar
  4. Diaz P, Señorans FJ, Reglero G, Ibañez E (2002) Truffle aromas analysis by headspace solid phase microextraction. J Agr Food Chem 50:6468–6472CrossRefGoogle Scholar
  5. Diaz P, Ibañez E, Señorans FJ, Reglero G (2003) Truffle aroma characterisation by headspace solid phase microextraction. J Chromatogr A 1017:207–214CrossRefGoogle Scholar
  6. DIRECTIVE 2000/13/EC OF THE EUROPEAN PARLIAMENT AND OF THE COUNCIL of 20 March 2000 on the approximation of the laws of the Member States relating to the labelling, presentation and advertising of foodstuffs. Official Journal of the European Communities L109: 29–42. Last accessed online 19/12/2014 at http://eur-lex.europa.eu/legal-content/EN/TXT/PDF/?uri=CELEX:32000L0013&from=EN
  7. Gioacchini A, Menotta M, Bertini L et al (2005) Solid-phase microextraction gas chromatography/mass spectrometry: a new method for species identification of truffles. Rapid Commun Mass Sp 19:2365–2370CrossRefGoogle Scholar
  8. Gioacchini A, Menotta M, Guescini M et al (2008) Geographical traceability of Italian white truffle (T. magnatum Pico) by analysis of volatile organic compounds. Rapid Commun Mass Sp 22:3147–3153CrossRefGoogle Scholar
  9. International Organisation of the Flavour Industry (2012) IOFI Code of Practice version 1.3. Last accessed online on 19/12/2014 at http://www.iofi.org/Home/Code-of-Practice/IOFI-Code-of-Practice/page.aspx/103
  10. Kataoka H, Lord HL, Pawliszyn J (2000) Applications of solid-phase microextraction in food analysis. J Chromatogr A 880:35–62CrossRefGoogle Scholar
  11. Kitson FG, Larsen BS, McEwen CN (1996) Gas chromatography and mass spectrometry: a practical guide. Academic, San Diego, pp. 3-35–325-365 Chapters 1 & 4Google Scholar
  12. March R, Richards D, Ryan R (2006) Volatile compounds from six species of truffle—head-space analysis and vapor analysis at high mass resolution. In J Mass Spectrom 249:60–67CrossRefGoogle Scholar
  13. NIST 08 (2008) Mass spectral library (NIST/EPA/NIH). Gaithersburg, MD, National Institute of Standards and Technology.Google Scholar
  14. Pacioni G, Cerretani L, Procida G, Cichelli A (2014) Composition of commercial truffle flavoured oils with GC-MS analysis and discrimination with an electronic nose. Food Chem 146:30–35CrossRefGoogle Scholar
  15. REGULATION (EC) No 1334/2008 OF THE EUROPEAN PARLIAMENT AND OF THE COUNCIL of 16 December 2008 on flavourings and certain food ingredients with flavouring properties for use in and on foods and amending Council Regulation (EEC) No 1601/91, Regulations (EC) No 2232/96 and (EC) No 110/2008 and Directive 2000/13/EC. Official Journal of the European Communities L354: 34–50. Last accessed online 19/12/2014 at http://www.effa.eu/media/pages/21/506/FL0905Regulation13342008EPCouncilFlavourings31122008.pdf
  16. REGULATION (EU) No 1169/2011 OF THE EUROPEAN PARLIAMENT AND OF THE COUNCIL of 25 October 2011 on the provision of food information to consumers, amending Regulations (EC) No 1924/2006 and (EC) No 1925/2006 of the European Parliament and of the Council, and repealing Commission Directive 87/250/EEC, Council Directive 90/496/EEC, Commission Directive 1999/10/EC, Directive 2000/13/EC of the European Parliament and of the Council, Commission Directives 2002/67/EC and 2008/5/EC and Commission Regulation (EC) No 608/2004. Official Journal of the European Union L304: 18–63. Last accessed online on 19/12/2014 at http://eur-lex.europa.eu/LexUriServ/LexUriServ.do?uri=OJ:L:2011:304:0018:0063:EN:PDF
  17. Rizzello R, Zampieri E, Vizzini A et al (2012) Authentication of prized white and black truffles in processed products using quantitative real-time PCR. Food Res Int 48:792–797CrossRefGoogle Scholar
  18. Splivallo R, Ottonello S, Mello A, Karlovsky P (2010) Truffle volatiles: from chemical ecology to aroma biosynthesis. New Phytol 189:688–699CrossRefGoogle Scholar
  19. Wang S, Marcone MF (2011) Biochemical and biological properties of the world’ most expensive underground edible mushroom: truffles. Food Res Int 44:2567–2581CrossRefGoogle Scholar

Copyright information

© Springer Science+Business Media New York 2016

Authors and Affiliations

  • Elisabetta Torregiani
    • 1
  • Sophie Lorier
    • 1
  • Gianni Sagratini
    • 1
  • Filippo Maggi
    • 1
  • Sauro Vittori
    • 1
  • Giovanni Caprioli
    • 1
  1. 1.School of PharmacyUniversity of CamerinoCamerinoItaly

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