Establishment of an Aqueous PEG 200-Based Deep Eutectic Solvent Extraction and Enrichment Method for Pumpkin (Cucurbita moschata) Seed Protein
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A novel approach for ultrasound–microwave synergistic extraction (UMSE) of pumpkin seed protein was developed using aqueous poly (ethylene glycol) (PEG 200)-based deep eutectic solvent (DES) as a green extraction medium. Key factors controlling the extraction and optimal operating conditions were optimized by combining the one variable at a time and response surface methodology. Results showed that the PEG 200 as a hydrogen bond donor combined with choline chloride as a typical hydrogen bond acceptor had a highest extraction efficiency among different solvents. The optimal extraction parameters were optimized as follows: PEG 200-based DES concentration, 28% w/w; solid to liquid ratio, 28 g mL−1; microwave power, 140 W; and extraction temperature, 43 °C. Under the optimal parameters, the actual extraction yield was 93.95 ± 0.23% (n = 3). The precipitation rate of pumpkin seed protein was 97.97% with a precipitation time of only 4 min by using an isoelectric point-ethanol-PEG 200 DES ternary co-precipitation method. Overall, this integrated method of PEG 200-based DES and UMSE exhibits a powerful tool for the rapid and efficient extraction of pumpkin seed protein.
KeywordsDeep eutectic solvent Polyethylene glycol Pumpkin seed protein Ultrasound–microwave synergistic extraction Response surface methodology
Compliance with Ethical Standards
The manuscript has not been published previously (partly or in full). The manuscript has not been submitted to more than one journal for simultaneous consideration.
Consent to submit has been received explicitly from all co-authors, as well as from the institute/organization where the work has been carried out before the work is submitted.
Authors whose names appear on the submission have contributed sufficiently to the scientific work and, therefore, share collective responsibility and accountability for the results.
The project were supported by the China Postdoctoral Science Foundation (No. 2016M592774) and the Open Projects Program of the Key Laboratory of Shaanxi Province Craniofacial Precision Medicine Research, Xi’an Jiaotong University (No. 2016LHM-KFKT002).
Conflict of Interest
Rui-Lin Liu declares that he has no conflict of interest. Pei Yu declares that she has no conflict of interest. Xian-Li Ge declares that he has no conflict of interest. Xiu-Feng Bai declares that he has no conflict of interest. Xing-Qiang Li declares that he has no conflict of interest. Qiang Fu declares that he has no conflict of interest.
This article does not contain any studies with human participants or animals performed by any of the authors.
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