Biorefinery Platform for Spathaspora passalidarum NRRL Y-27907 in the Production of Ethanol, Xylitol, and Single Cell Protein from Sugarcane Bagasse

BioEnergy Research (2021)Cite this article

Abstract

Spathaspora passalidarum is a naturally pentose-fermenting yeast with the potential to be applied for biotransformation of sugars from lignocellulosic biomasses. Despite being mostly investigated for ethanol production from sugarcane bagasse sugars (mainly xylose and glucose), this microorganism is also capable of producing high xylitol concentrations as a by-product of the ethanol fermentation. The integration of ethanol and xylitol production can improve the economic viability of the process due to the lower sale price of ethanol and the higher added-value of xylitol, a sucrose-substitute sugar with healthier properties. Considering the metabolic pathways interaction from glucose and xylose, it is essential to understand the effect of different glucose and xylose concentrations in the production of ethanol and xylitol by S. passalidarum. In this way, a simultaneous production of both products for the development of an integrated platform for food and chemical and biofuel industries can be accessed. Yeast biomass can also be recovered and applied as a protein source. Therefore, in this study, the fermentative performance of S. passalidarum was investigated in batch fermentations with different xylose and glucose concentrations, using synthetic substrate. The ATP levels and the enzymatic activities of xylose reductase (XR), xylitol dehydrogenase (XDH), and alcohol dehydrogenase (ADH) were also determined for each condition. The results indicated that low amounts of glucose (35%) were necessary to promote a higher xylitol production (10.58 ± 0.29 g/L) without losses on ethanol yield (78.99 ± 4.41%), being the most interesting condition for simultaneous formation of both products.

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Acknowledgements

The authors acknowledge the Brazilian Biorenewables National Laboratory/Brazilian Center of Research in Energy and Materials (LNBR/CNPEM).

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Affiliations

  1. School of Chemical Engineering, State University of Campinas – UNICAMP, 500 Albert Einstein Av, Campinas, SP, 13083-852, Brazil

    Carolina I. D. G. Bonan

  2. Brazilian Biorenewables National Laboratory – LNBR/CNPEM, 10000 Giuseppe Maximo Scolfaro St, Campinas, SP, 13083-100, Brazil

    Carolina I. D. G. Bonan, Robson Tramontina, Lauren B. Soares, Zaira B. Hoffmam, Natalia Coutouné & Fabio M. Squina

  3. Institute of Biology, State University of Campinas – UNICAMP, 255 Monteiro Lobato St, Campinas, SP, 13083-862, Brazil

    Robson Tramontina & Natalia Coutouné

  4. Chemical Engineering and Food Engineering Department, Federal University of Santa Catarina – UFSC, Florianópolis, SC, 88040-900, Brazil

    Monick W. dos Santos, Luiz E. Biazi, Lauren B. Soares, Isabela O. Pereira & Jaciane L. Ienczak

  5. Program of Technological and Environmental Processes, University of Sorocaba, Raposo Tavares Road, km 92.5 - Vila Artura, Sorocaba, SP, 18023-000, Brazil

    Fabio M. Squina

  6. Department of Microbiology, Immunology and Parasitology, Federal University of Santa Catarina – UFSC, Florianópolis, SC, 88040-900, Brazil

    Diogo Robl

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  1. Carolina I. D. G. Bonan
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Bonan, C.I.D.G., Tramontina, R., dos Santos, M.W. et al. Biorefinery Platform for Spathaspora passalidarum NRRL Y-27907 in the Production of Ethanol, Xylitol, and Single Cell Protein from Sugarcane Bagasse. Bioenerg. Res. (2021). https://doi.org/10.1007/s12155-021-10255-7

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Keywords

  • Biofuels
  • Yeast
  • Enzymatic activity
  • Alcohol dehydrogenase
  • Biomass