Abstract
In the present study, the effect of trace metal nutrients on biomass and lipid productivity of Scenedesmus obliquus cultured in municipal wastewater was evaluated. Lipid productivity of S. obliquus significantly increased by 18.63%, 19.21%, and 27.95% by adding trace metals of Mo, Mn, and Fe, respectively, with the highest lipid productivity of 24.69 mg/(L·d) in case of Fe (3.00 mg/L FeSO4·7H2O). GC analysis of the lipids showed that the control (WW) and ferrous sulfate–treated cultures had very similar compositions, and both of them contained considerable proportions C16-18, showing their suitability for biodiesel production. Moreover, using FeSO4·7H2O enhanced the removal rates of total phosphorus, ammonia nitrogen, and total nitrogen in municipal wastewater, which attained 94.7%, 99.2%, and 95.8%, respectively.
References
Han S, Jin W, Tu R, Wu W (2015) Biofuel production from microalgae as feedstock: current status and potential. Crit Rev Biotechnol 35:255–268
Muthuraj M, Chandra N, Palabhanvi B, Kumar V, Das D (2015) Process engineering for high-cell-density cultivation of lipid rich microalgal biomass of Chlorella sp FC2 IITG. Bioenerg Res 8:726–739
Wu Y, Yu Y, Hu H (2014) Effects of initial phosphorus concentration and light intensity on biomass yield per phosphorus and lipid accumulation of Scenedesmus sp LX1. Bioenerg Res 7:927–934
Zhou WG, Chen P, Min M, Ma XC, Wang JH, Griffith R, Hussain F, Peng P, Xie QL, Li Y (2014) Environment-enhancing algal biofuel production using wastewaters. Renew Sust Energ Rev 36:256–269
Yang J, Xu M, Zhang X, Hu Q, Sommerfeld M, Chen Y (2011) Life-cycle analysis on biodiesel production from microalgae: water footprint and nutrients balance. Bioresour Technol 102:159–165
Tu RJ, Jin WB, Xi TT, Yang Q, Han SF, Abomohra A (2015) Effect of static magnetic field on the oxygen production of Scenedesmus obliquus cultivated in municipal wastewater. Water Res 86:132–138
Lee CS, Oh HS, Oh HM, Kim HS, Ahn CY (2016) Two-phase photoperiodic cultivation of algal-bacterial consortia for high biomass production and efficient nutrient removal from municipal wastewater. Bioresour Technol 200:867–875
Cho DH, Choi JW, Kang Z, Kim BH, Oh HM, Kim HS, Ramanan R (2017) Microalgal diversity fosters stable biomass productivity in open ponds treating wastewater. Sci Rep-Uk 7
Han SF, Jin WB, Tu RJ, Abomohra A, Wang ZH (2016) Optimization of aeration for biodiesel production by Scenedesmus obliquus grown in municipal wastewater. Bioprocess Biosyst Eng 39:1073–1079
Han SF, Jin WB, Chen YG, Tu RJ, Abomohra A (2016) Enhancement of lipid production of Chlorella Pyrenoidosa cultivated in municipal wastewater by magnetic treatment. Appl Biochem Biotech 180:1043–1055
Yu Z, Song M, Pei H, Jiang L, Hou Q, Nie C, Zhang L (2017) The effects of combined agricultural phytohormones on the growth, carbon partitioning and cell morphology of two screened algae. Bioresour Technol 239:87–96
Park W, Yoo G, Moon M, Kim CW, Choi Y, Yang J (2013) Phytohormone supplementation significantly increases growth of Chlamydomonas reinhardtii cultivated for biodiesel production. Appl Biochem Biotech 171:1128–1142
Ren H, Liu B, Kong F, Zhao L, Xie G, Ren N (2014) Enhanced lipid accumulation of green microalga Scenedesmus sp by metal ions and EDTA addition. Bioresour Technol 169:763–767
Che R, Huang L, Yu X (2015) Enhanced biomass production, lipid yield and sedimentation efficiency by iron ion. Bioresour Technol 192:795–798
Salama ES, Kurade MB, Abou-Shanab R, El-Dalatony MM, Yang IS, Min B, Jeon BH (2017) Recent progress in microalgal biomass production coupled with wastewater treatment for biofuel generation. Renew Sust Energ Rev 79:1189–1211
Abomohra AE, Wagner M, El-Sheekh M, Hanelt D (2013) Lipid and total fatty acid productivity in photoautotrophic fresh water microalgae: screening studies towards biodiesel production. J Appl Phycol 25:931–936
Folch J, Lees M, Stanley GA (1957) Simple method for the isolation and purification of total lipids from animal tissues. J Biol Chem 226:497–509
Kaczmarzyk D, Fulda M (2010) Fatty acid activation in cyanobacteria mediated by acyl-acyl carrier protein synthetase enables fatty acid recycling. Plant Physiol 152:1598–1610
State Environmental Protection Administration (2002) Water and wastewater monitoring analysis method. China Environmental Science Press, Beijing
Sunda WG, Huntsman SA (1995) Iron uptake and growth limitation in oceanic and coastal phytoplankton. Mar Chem 50:189–206
Chen M, Tang H, Ma H, Holland TC, Ng KYS, Salley SO (2011) Effect of nutrients on growth and lipid accumulation in the green algae Dunaliella tertiolecta. Bioresour Technol 102:1649–1655
Hider RC, Kong X (2010) Chemistry and biology of siderophores. Nat Prod Rep 27:637–657
Li X, Hu HY, Gan K, Sun YX (2010) Effects of different nitrogen and phosphorus concentrations on the growth, nutrient uptake, and lipid accumulation of a freshwater microalga Scenedesmus sp. Bioresour Technol 101:5494–5500
Hoekman SK, Broch A, Robbins C, Ceniceros E, Natarajan M (2012) Review of biodiesel composition, properties, and specifications. Renew Sust Energ Rev 16:143–169
Song YH, Hahn HH, Hoffmann E (2002) Effects of solution conditions on the precipitation of phosphate for recovery - a thermodynamic evaluation. Chemosphere 48:1029–1034
Richmond (Ed.), (2004) Handbook of microalgal culture. Blackwell Science Ltd., Oxford, UK
Hoffmann JP (1998) Wastewater treatment with suspended and nonsuspended algae. J Phycol 34(5):757–763
Kim BH, Kang Z, Ramanan R, Choi JE, Cho DH, Oh HM, Kim HS (2014) Nutrient removal and biofuel production in high rate algal pond using real municipal wastewater. J Microbiol Biotechnol 24:1123–1132
Reeves TG (1972) Nitrogen removal - literature review. J Water Pollut Control Fed 44:1895
Matusiak K, Przytockajusiak M, Leszczynskagerula K, Horoch M (1976) Studies on purification of wastewater from nitrogen-fertilizer industry by intensive algal cultures .2. Removal of nitrogen from wastewater. Acta Microbiol Pol 25:361–374
Park WK, Yoo G, Moon M, Kim CW, Choi YE, Yang JW (2013) Phytohormone supplementation significantly increases growth of Chlamydomonas reinhardtii cultivated for biodiesel production. Appl Biochem Biotech 171(5):1128–1142
Funding
This work was supported by the National Natural Science Foundation of China (No. 51878215), Natural Science Foundation of Guangdong Province, China (2018A030313185), and Shenzhen Science and Technology Innovation Project (KJYY20171011144235970, JCYJ20170307150223308).
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Han, SF., Jin, W., Abomohra, A.EF. et al. Municipal Wastewater Enriched with Trace Metals for Enhanced Lipid Production of the Biodiesel-Promising Microalga Scenedesmus obliquus. Bioenerg. Res. 12, 1127–1133 (2019). https://doi.org/10.1007/s12155-019-10042-5
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DOI: https://doi.org/10.1007/s12155-019-10042-5