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The potential for clinical applications using a new ionization method combined with ion mobility spectrometry-mass spectrometry

  • Original Research
  • Published:
International Journal for Ion Mobility Spectrometry

Abstract

Recently discovered ionization methods for use in mass spectrometry (MS), are widely applicable to biological materials, robust, and easy to automate. Among these, matrix assisted ionization vacuum (MAIV) is astonishing in that ionization of low and high-mass compounds are converted to gas-phase ions with charge states similar to electrospray ionization simply by exposing a matrix:analyte mixture to the vacuum of a mass spectrometer. Using the matrix compound, 3-nitrobenzonitrile, abundant ions are produced at room temperature without the need of high voltage or a laser. Here we discuss chemical analyses advances using MAIV combined with ion mobility spectrometry (IMS) real time separation, high resolution MS, and mass selected and non-mass selected MS/MS providing rapid analyte characterization. Drugs, their metabolites, lipids, peptides, and proteins can be ionized simultaneously from a variety of different biological matrixes such as urine, plasma, whole blood, and tissue. These complex mixtures are best characterized using a separation step, which is obtained nearly instantaneously with IMS, and together with direct ionization and MS or MS/MS provides a fast analysis method that has considerable potential for non-targeted clinical analyses.

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Acknowledgement

The authors are grateful for funding and support from Wayne State University (Schaap, Summer, and Rumble Dissertation Fellowships to EDI, and Schaap Faculty Scholar to ST), NSF CAREER Award 0955975, ASMS Research Award, DuPont Young Professor Award, Waters Center of Innovation Award, and Eli Lilly Young Investigator Award in Analytical Chemistry (to ST), as well as NIH DA011322 and DA021696 (to KM). The authors are thankful to Keith Richardson from Waters Co. for the data analysis using BayesSpray software.

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Authors and Affiliations

  1. Department of Chemistry, Wayne State University, Detroit, MI, 48202, USA

    Ellen D. Inutan & Sarah Trimpin

  2. Gill Center for Biomolecular Science, Indiana University, Bloomington, IN, 47405, USA

    James Wager-Miller & Ken Mackie

  3. Detroit Medical Center University Laboratories, Detroit, MI, 48219, USA

    Srinivas B. Narayan

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  1. Ellen D. Inutan
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  2. James Wager-Miller
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  3. Srinivas B. Narayan
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  4. Ken Mackie
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  5. Sarah Trimpin
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Correspondence to Sarah Trimpin.

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Inutan, E.D., Wager-Miller, J., Narayan, S.B. et al. The potential for clinical applications using a new ionization method combined with ion mobility spectrometry-mass spectrometry. Int. J. Ion Mobil. Spec. 16, 145–159 (2013). https://doi.org/10.1007/s12127-013-0131-7

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  • DOI: https://doi.org/10.1007/s12127-013-0131-7

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