Chemical shift assignments of RHE_RS02845, a NTF2-like domain-containing protein from Rhizobium etli
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The nuclear transport factor 2 (NTF2) like superfamily includes members of the NTF2 family, delta-5-3-ketosteroid isomerases, and the beta subunit of ring hydroxygenases. This family plays important roles in both eukaryotic and prokaryotic cells, and is taken as a classic example of divergent evolution because proteins in this family exhibit diverse biological functions, although share common structural features. We cloned the gene RHE_RS02845 encoding a predicted NTF2-like domain-containing protein in Rhizobium etli, and prepared U-13C/15N-labeled protein samples for its three-dimensional NMR structural determination. Here, chemical shift assignments for both backbone and side-chain atoms are reported, which is prerequisite for further structural calculation and functional research using NMR spectroscopy.
KeywordsRHE_RS02845 NTF2-like domain Rhizobium etli CFN42 Chemical shift assignments
This work was supported by funds from the National Natural Sciences Foundation of China (Grant Number: 21575155) and the Hundred Talent Program by Chinese Academy of Sciences.
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Conflict of interest
The authors declare that there is no conflict of interest.
- Goddard TD, Kneller DG (2008) SPARKY 3. University of California, San FransiscoGoogle Scholar
- Gonzalez V, Santamaria RI, Bustos P, Hernandez-Gonzalez I, Medrano-Soto A, Moreno-Hagelsieb G, Janga SC, Ramirez MA, Jimenez-Jacinto V, Collado-Vides J, Davila G (2006) The partitioned Rhizobium etli genome: genetic and metabolic redundancy in seven interacting replicons. Proc Natl Acad Sci USA 103:3834–3839ADSCrossRefGoogle Scholar