Advertisement

Biomolecular NMR Assignments

, Volume 12, Issue 1, pp 159–161 | Cite as

1H, 13C and 15N resonance assignments of NEDD8 from Trypanosoma brucei

  • Rui Wang
  • Jiahai Zhang
  • Shanhui Liao
  • Xiaoming Tu
Article
  • 60 Downloads

Abstract

Neural precursor cell-expressed, developmently downregulated 8 (NEDD8) is a small ubiquitin-like modifier, which plays important roles in many cellular processes. Although it has been well studied in many eukaryotes, NEDD8 is still uncharacterized in some unicellular parasites, such as Trypanosoma brucei (T. brucei). Here we report the resonance assignments of NEDD8 from T. brucei.

Keywords

NEDD8 T. brucei Resonance assignment 

Notes

Acknowledgements

We thank F. Delaglio and A. bax for providing NMRPipe and NMRDraw and also thank Goddard and Kneller for providing SPARKY software. This work was supported by the Chinese National Natural Science Foundation (Grant Number 31270780, U1332137 and 31500601), and the Doctoral Research Program of Henan University of Science and Technology, grant number 4020/13480038.

References

  1. Delaglio F, Grzesiek S, Vuister GW, Zhu G, Pfizer J, Bax A (1995) NMRPipe: a multidimensional spectral processing system based on UNIX pipes. J Biomol NMR 6:277–293CrossRefGoogle Scholar
  2. Enchev RI, Schulman BA, Peter M (2015) Protein neddylation: beyond cullin-RING ligases. Nat Rev Mol Cell Biol 16(1):30–44CrossRefGoogle Scholar
  3. Goddard TD, Kneller DG (1993) SPARKY3. University of California, San FranciscoGoogle Scholar
  4. Higa LA, Mihaylov IS, Banks DP, Zheng J, Zhang H (2003) Radiation-mediated proteolysis of CDT1 by CUL4-ROC1 and CSN complexes constitutes a new checkpoint. Nat Cell Biol 5:1008–1015CrossRefGoogle Scholar
  5. Jia L, Sun Y (2011) SCF E3 ubiquitin ligases as anticancer targets. Curr Cancer Drug Targets 11:347–356CrossRefGoogle Scholar
  6. Koopmann R, Muhammad K, Perbandt M, Betzel C, Duszenko M (2009) Trypanosoma brucei ATG8: structural insights into autophagic-like mechanisms in protozoa. Autophagy 5(8):1085–1091CrossRefGoogle Scholar
  7. Li H, Zhu H, Liu Y, He F, Xie P, Zhang L (2016) Itch promotes the neddylation of JunB and regulates JunB-dependent transcription. Cell Signal 28:1186–1195CrossRefGoogle Scholar
  8. Liao S, Wang T, Fan K, Tu X (2010) The small ubiquitin-like modifier (SUMO) is essential in cell cycle regulation in Trypanosoma brucei. Exp Cell Res 316(5):704–715CrossRefGoogle Scholar
  9. Liao S, Hu H, Wang T, Tu X, Li Z (2017) The protein neddylation pathway in Trypanosoma brucei: functional characterization and substrate identification. J Biol Chem 292(3):1081–1091CrossRefGoogle Scholar
  10. Nawrocki ST, Griffin P, Kelly KR, Carew JS (2012) MLN4924: a novel first-in-class inhibitor of NEDD8-activating enzyme for cancer therapy. Exp Opin Investig Drugs 21(10):1563–1573CrossRefGoogle Scholar
  11. Petroski MD, Deshaies RJ (2005) Function and regulation of cullin-RING ubiquitin ligases. Nat Rev Mol Cell Biol 6:9–20CrossRefGoogle Scholar
  12. Soucy TA, Smith PG, Milhollen MA, Berger AJ, Gavin JM et al (2009) An inhibitor of NEDD8-activating enzyme as a new approach to treat cancer. Nature 458(7239):732–736ADSCrossRefGoogle Scholar
  13. Wei N, Deng XW (2003) The COP9 signalosome. Annu Rev Cell Dev Biol 19:261–286CrossRefGoogle Scholar
  14. Wishart DS, Sykes BD (1994) The 13C chemical-shift index: a simple method for the identification of protein secondary structure using 13C chemical-shift data. J Biomol NMR 4:171–180CrossRefGoogle Scholar
  15. Xirodimas DP, Saville MK, Bourdon JC, Hay RT, Lane DP (2004) Mdm2-mediated NEDD8 conjugation of p53 inhibits its transcriptional activity. Cell 118:83–97CrossRefGoogle Scholar
  16. Zhang W, Zhang J, Xu C, Wang T, Zhang X, Tu X (2009) Solution structure of Urm1 from Trypanosoma brucei. Proteins 75(3):781–785CrossRefGoogle Scholar

Copyright information

© Springer Science+Business Media B.V., part of Springer Nature 2018

Authors and Affiliations

  1. 1.Department of Anthropotomy and Histoembryology, Medical CollegeHenan University of Science and TechnologyLuoyangPeople’s Republic of China
  2. 2.Hefei National Laboratory for Physical Sciences at Microscale, and School of Life SciencesUniversity of Science and Technology of ChinaHefeiPeople’s Republic of China

Personalised recommendations