Epidemiology of Acute Gastroenteritis Caused by Rotavirus among Children Less than Five Years Old Admitted in Hospital, in North India



To report the data of burden of rotaviral acute gastroenteritis in under-five children from two states post-introduction of the vaccine.


Children under 5 y of age hospitalized with diarrhea from the states of Haryana and Himachal Pradesh in north India were recruited in the study. Commercially available ELISA kits were used for testing rotavirus in the collected stool samples. Genotyping of the positive samples was done by reverse-transcription polymerase chain reaction.


Out of 345 samples collected, 69 (20%) were found to be positive for rotavirus by ELISA. Genotyping was done and G3P[8] (31.3%), G1P[8] (13.4%), G2P[4] (13.4%) were found to be prevalent strains. Mixed strains were also found in 19.4% stool samples.


The study highlighted the high burden of rotavirus associated diarrhea in north Indian states. The data is helpful for evaluating the impact of vaccine on the severity of acute gastroenteritis and the changing strains after the introduction of rotavirus vaccine in the Universal Immunization Program.

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The study was supported by the Indian Council of Medical Research (ICMR), New Delhi; Ministry of Health and Family Welfare (MoHFW), Govt. of India and the State government of the participating institution. The authors thank the team at CMC, Vellore for support, and the children and families for participation.


Subcontract to CMC, Vellore via grant no MOA#871-15SC and the Translational Health Science and Technology Institute (grant no OPP1165083) was provided by Centers for Disease Control and Prevention, Atlanta, GA, USA which was funded by Bill and Melinda Gates Foundation for conducting the study.

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Conception and design of the study – MG, NPN; Acquisition of data – AB, SA, MR, AB, JM, AS; Analysis of data – MG, AB, VT; Interpretation of data – MG, AB, NPN; Drafting of article – MG, AB, NPN; All authors critically revised and accepted the final version of the manuscript. MG will act as guarantor for this paper.

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Correspondence to Madhu Gupta.

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Ethical approval for the study was taken from the Institute Ethics Committee, PGIMER, Chandigarh.

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Gupta, M., Bansal, A., Bansal, A. et al. Epidemiology of Acute Gastroenteritis Caused by Rotavirus among Children Less than Five Years Old Admitted in Hospital, in North India. Indian J Pediatr 88, 22–27 (2021). https://doi.org/10.1007/s12098-020-03619-y

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  • Acute gastroenteritis
  • Rotavirus
  • Rotavirus vaccine
  • Epidemiology
  • Surveillance