Expression levels of SCCA and CYFRA 21-1 in serum of patients with laryngeal squamous cell carcinoma and their correlation with tumorigenesis and progression



To explore the concentration of squamous cell carcinoma antigen (SCCA), cytokeratin fragment antigen 21-1 (CYFRA21-1) in patients with laryngeal squamous cell carcinoma (LSCC) and its correlation with tumorigenesis and progression.


A total of 78 patients with LSCC admitted to our hospital from February 2010 to January 2016 were enrolled as the research group (RG), and another 41 healthy volunteers from the same period were selected as the control group (CG). The serum concentrations of SCCA and CYFRA21-1 in patients with LSCC were detected by ELISA, whose diagnostic value in LSCC were further analyzed by ROC curve. The prognosis and survival curves of patients with LSCC were observed according to the median value of serum SCCA and CYFRA21-1 concentrations.


The concentration of CYFRA21-1 and SCCA in the RG was significantly higher than that in the CG (p < 0.050). The SCCA and CYFRA21-1 identified a significant difference in smoking, lymphatic metastasis, TNM staging, and differentiation degree (p < 0.050). The survival rate of the SCCA low-concentration group was significantly better than that of the high-concentration group, p < 0.050. The survival rate of the CYFRA21-1 low-concentration group was markedly better than that of the high-concentration group, p < 0.050.


SCCA and CYFRA21-1 are highly concentrated in LSCC patients, which have good diagnostic efficacy for LSCC. In addition, they play some certain role in the occurrence and development of LSCC, and are expected to be markers for early diagnosis and prognosis of this disease.

This is a preview of subscription content, log in to check access.

Fig. 1
Fig. 2
Fig. 3


  1. 1.

    Chu EA, Kim YJ. Laryngeal cancer: diagnosis and preoperative work-up. Otolaryngol Clin North Am. 2008;41:673–95.

    Article  Google Scholar 

  2. 2.

    Blot WJ, McLaughlin JK, Winn DM, et al. Smoking and drinking in relation to oral and pharyngeal cancer. Cancer Res. 1988;48:3282–7.

    CAS  PubMed  Google Scholar 

  3. 3.

    Argiris A, Karamouzis MV, Raben D, et al. Head and neck cancer. Lancet. 2008;371:1695–709.

    CAS  Article  Google Scholar 

  4. 4.

    Marchiano E, Patel DM, Patel TD, et al. Subglottic squamous cell carcinoma: a population-based study of 889 cases. Otolaryngol Head Neck Surg. 2016;154:315–21.

    Article  Google Scholar 

  5. 5.

    Greenlee RT, Hill-Harmon MB, Murray T, et al. Cancer statistics, 2001. CA Cancer J Clin 2001;51: 15–36. CA Cancer J Clin. 2001;2001(51):144.

    Google Scholar 

  6. 6.

    Torrente MC, Rodrigo JP, Haigentz M Jr, et al. Human papillomavirus infections in laryngeal cancer. Head Neck. 2011;33:581–6.

    Article  Google Scholar 

  7. 7.

    De Foer B, Hermans R, Van der Goten A, et al. Imaging features in 35 cases of submucosal laryngeal mass lesions. Eur Radio. 1996;6:913–9.

    Article  Google Scholar 

  8. 8.

    Saleh EM, Mancuso AA, Stringer SP. CT of submucosal and occult laryngeal masses. J Comput Assist Tomogr. 1992;16:87–93.

    CAS  Article  Google Scholar 

  9. 9.

    Task Force, Ahn SH, Hong HJ, et al. Guidelines for the surgical management of laryngeal cancer: Korean Society of Thyroid-Head and Neck Surgery. Clin Exp Otorhinolaryngol. 2017;10:1.

    Article  Google Scholar 

  10. 10.

    Li X, Zhou J, Huang K, et al. The predictive value of serum squamous cell carcinoma antigen in patients with cervical cancer who receive neoadjuvant chemotherapy followed by radical surgery: a single-institute study. PLoS ONE. 2015;10:e0122361.

    Article  Google Scholar 

  11. 11.

    Kulpa J, Wójcik E, Reinfuss M, et al. Carcinoembryonic antigen, squamous cell carcinoma antigen, CYFRA 21-1, and neuron-specific enolase in squamous cell lung cancer patients. Clin Chem. 2002;48:1931–7.

    CAS  Article  Google Scholar 

  12. 12.

    Al-Shagahin H, Alkotyfan K, Müller HH, et al. Cyfra 21-1 as a serum tumor marker for follow-up of patients with laryngeal and hypopharyngeal squamous cell carcinoma. Anticancer Res. 2009;29:3421–5.

    CAS  PubMed  Google Scholar 

  13. 13.

    Silverberg E, Lubera JA. Cancer statistics, 1988. CA Cancer J Clin. 1988;38:5–22.

    CAS  Article  Google Scholar 

  14. 14.

    Hoffman HT, Porter K, Karnell LH, et al. Laryngeal cancer in the United States: changes in demographics, patterns of care, and survival. Laryngoscope. 2006;116:1–13.

    Article  Google Scholar 

  15. 15.

    Shah JP, Tollefsen HR. Epidermoid carcinoma of the supraglottic larynx: role of neck dissection in initial surgical treatment. Am J Surg. 1974;128:494–8.

    CAS  Article  Google Scholar 

  16. 16.

    Hawkins NV. The treatment of glottic carcinoma: an analysis of 800 cases. Laryngoscope. 1975;85:1485–93.

    CAS  Article  Google Scholar 

  17. 17.

    Wolf GT, Fisher SG, Hong WK, et al. Induction chemotherapy plus radiation compared with surgery plus radiation in patients with advanced laryngeal cancer. N Engl J Med. 1991;324:1685–90.

    Article  Google Scholar 

  18. 18.

    Zhao Q, Duan R, Yuan J, et al. A reusable localized surface plasmon resonance biosensor for quantitative detection of serum squamous cell carcinoma antigen in cervical cancer patients based on silver nanoparticles array. Int J Nanomed. 2014;9:1097.

    Google Scholar 

  19. 19.

    Kato H. Expression and function of squamous cell carcinoma antigen. Anticancer Res. 1996;16:2149–53.

    CAS  PubMed  Google Scholar 

  20. 20.

    Qu T, Zhang J, Xu N, et al. Diagnostic value analysis of combined detection of Trx, CYFRA21-1 and SCCA in lung cancer. Oncol Lett. 2019;17:4293–8.

    CAS  PubMed  PubMed Central  Google Scholar 

  21. 21.

    Yuan C, Yang K, Tang H, et al. Diagnostic values of serum tumor markers Cyfra21-1, SCCAg, ferritin, CEA, CA19-9, and AFP in oral/oropharyngeal squamous cell carcinoma. Onco Targets Ther. 2016;9:3381.

    CAS  PubMed  PubMed Central  Google Scholar 

Download references

Author information




MJ conceived the study and drafted the manuscript. LZ observed the indicators. Both authors read and approved the final manuscript.

Corresponding author

Correspondence to L. J. Zhang.

Ethics declarations

Conflict of interest

The authors declare that they have no conflict of interest.

Ethical approval

The study was approved by Ethical Committee of Affiliated Hospital of Weifang Medical University and conducted in accordance with the ethical standards.

Informed consent

All the subjects signed the informed consent.

Additional information

Publisher's Note

Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations.

Rights and permissions

Reprints and Permissions

About this article

Verify currency and authenticity via CrossMark

Cite this article

Ji, M., Zhang, L.J. Expression levels of SCCA and CYFRA 21-1 in serum of patients with laryngeal squamous cell carcinoma and their correlation with tumorigenesis and progression. Clin Transl Oncol (2020).

Download citation


  • Laryngeal squamous cell carcinoma
  • Squamous cell carcinoma antigen
  • CYFRA21-1
  • Prognosis
  • Diagnostic value