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Changes in Gene Expression in Human Epithelial and Mast Cells in Response to Vesicles from Klebsiella pneumonia ATCC 13883

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Abstract

We investigated alterations in the expression of immune-related genes in epithelial cells and mast cells treated with outer membrane vesicles (OMVs) derived from Klebsiella pneumoniae (KpOMVs). Previous studies have shown that OMVs contain substances that enable their delivery to host cells and induce an immune response. Our results indicate an increase in expression of genes such as IL-8, IL-1b, MIP-1α, HMOX1, HSPA1A, and IL-24 in epithelial cells and mast cells treated with KpOMVs. The pathogenicity of KpOMVs was confirmed by measuring the changes in the expression of these immune-related genes.

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Acknowledgements

This research was supported by the Basic Science Research Program, through the National Research Foundation of Korea (NRF), funded by the Ministry of Science ICT & Future Planning (NRF-2014R1A1A1002349).

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Authors and Affiliations

  1. Department of Biomedical Laboratory Science, School of Medicine, Eulji University, #143-5, Yongdu-dong, Jung-gu, Daejeon, 301-746, Republic of Korea

    Hee Sang You, Yeon Jeong Ok, Song Hee Lee & Sung Hee Hyun

  2. BK21 Plus Program, Department of Senior Healthcare, Graduate School, Eulji University, Daejeon, Republic of Korea

    Hee Sang You & Sung Hee Hyun

  3. School of Science Education, Chungbuk National University, Gaeshin-dong, Heungdok-gu, Chongju, Republic of Korea

    Sang Sun Kang

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  1. Hee Sang You
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  2. Yeon Jeong Ok
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Correspondence to Sung Hee Hyun.

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You, H.S., Ok, Y.J., Lee, S.H. et al. Changes in Gene Expression in Human Epithelial and Mast Cells in Response to Vesicles from Klebsiella pneumonia ATCC 13883. Indian J Microbiol 59, 241–245 (2019). https://doi.org/10.1007/s12088-018-0774-5

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  • DOI: https://doi.org/10.1007/s12088-018-0774-5

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