Investigating the Role of Endophytic Fungi in Gentiana scabra bge. by Cross-Growth Period Inoculation
Gentiana scabra Bge. (gentian) is a Chinese medicinal plant. Endophytic fungi from the roots of gentian were isolated and cross-growth period inoculation was performed to study the roles of three Trichoderma spp. strains (F1, F2, and F9) in their original host plant. In treatments inoculated with F1, F2, and F9, gentiopicroside content increased 33.6, 23.7 and 13% than that in the control. Strains F1, F2, and F9 could also improve polysaccharide content by more than 6.6, 18.7 and 30% compared to the control. The incidence of spot blight in gentian inoculated with F1, F2, and F9 decreased by 31.2, 26.7 and 8.5%. Inconsistent changes in the activity of the three enzymes (superoxide dismutase, catalase and peroxidase) were observed when the plants were attacked by pathogens or inoculated with fungi. High enzymatic activity did not reflect mild disease. Cross-growth period inoculation, which takes into account the original living environment (gentian plant as “substrate” and different microorganisms as symbionts) of endophytic fungi, provides a new idea for studying effects of endophytes on their original hosts. This is the first research about the role of endophytic fungi in Gentiana scabra bge. in vivo.
KeywordsEndophytic fungi Gentiana scabra Bge. Growth-promoting Disease-resistant Defense enzyme
We are grateful to the National Natural Science Foundation of China (NSFC No. 31400441).
Compliance with ethical standards
Conflict of interest
The authors declare that they have no conflict of interest.
- 14.Cao FH, Li C (2008) New advantages in research of chemical constituents and pharmacological activities of genus Gentiana. Chin J New Drugs 17:27–29, 32Google Scholar
- 15.Yang SB, Wang C (2005) Research progress in chemical constituents and pharmacological activities of Radix gentianae. Acta Chin Med Pharm 33:54–56Google Scholar
- 16.Jiang WX, Zhao X (2008) Study on anticoagulant function of gentian polysaccharides. J Heilongjiang Med 5:31–32Google Scholar
- 17.Wang JH (2012) Research about the effects of gentian polysaccharides on liver protection, lipid decrease and immune regulation. M.S. thesis. College of Harbin Commerce University, HarbinGoogle Scholar
- 18.Du XW, Zhou YT (2010) Experimental research about antagonistic action of endophytic fungi in Gentiana manshurica on Gentian leaf blight. In: Abstract: 2nd national traditional Chinese medicine commodity conference. China Society of Commodity Science, pp 104–105Google Scholar
- 19.Song YQ, Liu WH (2017) Isolation and antimicrobial activity of endophytic fungi from Yunnan Gentiana. J Qujing Normal Univ 36:33–36Google Scholar
- 26.Krauss J, Harri DA, Bush L, Power SA, Muller CB (2007) Fungal grass endophytes, grass cultivars, nitrogen deposition and the associations with colonizing insects. In: 6th international symposium on fungal endophytes of grasses, “From Lab To Farm”, Christchurch, pp 53–57Google Scholar
- 27.White TJ, Bruns T, Lee S, Taylor J (1990) Analysis of phylogenetic relationships by amplification and direct sequencing of ribosomal RNA genes. In: Innis MA, Gefland DH, Sninsky JJ, White TJ (eds) PCR protocols: a guide to methods and applications. Academic Press, New York, pp 315–322Google Scholar
- 28.Chinese Pharmacopoeia Commission (2015) Pharmacopoeia of the People’s Republic of China part 1. Medical Science and Technology Press, Beijing, p 96Google Scholar
- 29.Duan Y, Wang DM, Pan YN, Lu Y, Wu XL, Liu XQ (2014) Correlation between the accumulation of polysaccharide and gentiopicroside in Gentianae Radix et Rhizoma and soil factors from Liaoning GAP base. J Shenyang Pharm Univ 31:998–1002Google Scholar
- 30.Liu JH, Li J, Zhang YQ (2010) Germination characteristics and secondary metabolism regulation of Scutellaria baicalensis Georgi seeds under different temperatures. Agric Sci Technol 11:213–216Google Scholar