Advertisement

Indian Journal of Microbiology

, Volume 58, Issue 3, pp 301–311 | Cite as

Comparison of Antimicrobial Properties of Silver Nanoparticles Synthesized from Selected Bacteria

  • M. M. K. Peiris
  • S. S. N. Fernando
  • P. M. Jayaweera
  • N. D. H. Arachchi
  • T. D. C. P. Guansekara
Original Research Article

Abstract

Green silver nanoparticle (AgNP) biosynthesis is facilitated by the enzyme mediated reduction of Ag ions by plants, fungi and bacteria. The antimicrobial activity of green AgNPs is useful to overcome the challenge of antimicrobial resistance. Antimicrobial properties of biosynthesized AgNPs depend on multiple factors including culture conditions and the microbial source. The antimicrobial activity of AgNPs biosynthesized by Pseudomonas aeruginosa ATCC 27853, Escherichia coli ATCC 25922, Staphylococcus aureus ATCC 25923 and Acinetobacter baumannii (confirmed clinical isolate) were investigated in this study. Biosynthesis conditions (AgNO3 concentration, pH, incubation temperature and incubation time) were optimized to obtain the maximum AgNP yield. Presence of AgNPs was confirmed by observing a characteristic UV–Visible absorbance peak in 420–435 nm range. AgNP biosynthesis was optimal at 0.4 g/L AgNO3 concentration under alkaline conditions at 60–70 °C. The biosynthesized AgNPs showed higher stability compared to chemogenized AgNPs in the presence of electrolytes. AgNPs synthesized by P. aeruginosa were the most stable while NPs of S. aureus were the least stable. AgNPs synthesized by P. aeruginosa and S. aureus showed good antimicrobial potential against E. coli, P. aeruginosa, S. aureus, MRSA and Candida albicans. AgNPs synthesized by S. aureus had greater antimicrobial activity. The antimicrobial activity of NPs may vary depending on the size and the morphology of NPs.

Keywords

Silver nanoparticle Antimicrobial activity UV–Visible spectroscopy Colony forming units TEM 

Abbreviations

AgNPs

Silver nanoparticles

ZOI

Zone of inhibition

CFU

Colony forming units

Notes

Acknowledgements

The authors acknowledge the financial support (Grant No. ASP/01/RE/MED/2016/42) and laboratory facilities provided by the University of Sri Jayewardenepura.

References

  1. 1.
    Oza G, Pandey S, Shah R, Sharon M (2012) Extracellular fabrication of silver nanoparticles using Pseudomonas aeruginosa and its antimicrobial assay. Pelagia Res Lib Adv Appl Sci Res 3:1776–1783. https://www.researchgate.net/profile/Dr_Sunil_Pandey2/publication/230707314_Extracellular_Fabrication of Silver Nanoparticles using Pseudomonas aeruginosa and its Antimicrobial Assay/links/0912f5034 8c53f0b10000000.pdf. Accessed 27 Aug 2017
  2. 2.
    Li X, Xu H, Chen Z-S, Chen G (2011) Biosynthesis of nanoparticles by microorganisms and their applications. J Nanomater 2011:1–16.  https://doi.org/10.1155/2011/270974 Google Scholar
  3. 3.
    Otari SV, Patel SK, Jeong J-H, Lee JH, Lee J-K (2016) A green chemistry approach for synthesizing thermostable antimicrobial peptide-coated gold nanoparticles immobilized in an alginate biohydrogel. RSC Adv 6:86808–86816.  https://doi.org/10.1039/C6RA14688K CrossRefGoogle Scholar
  4. 4.
    Hölken I, Hoppe M, Mishra YK (2016) Complex shaped ZnO nano-and microstructure based polymer composites: mechanically stable and environmentally friendly coatings for potential antifouling applications. Phys Chem Chem Phys 18:7114–7123.  https://doi.org/10.1039/C5CP07451G CrossRefPubMedGoogle Scholar
  5. 5.
    Hunagund SM, Desai VR, Barretto DA, Pujar MS, Kadadevarmath JS, Vootla S, Sidarai AH (2017) Photocatalysis effect of a novel green synthesis gadolinium doped titanium dioxide nanoparticles on their biological activities. J Photochem Photobiol 346:159–167.  https://doi.org/10.1016/j.jphotochem.2017.06.003 CrossRefGoogle Scholar
  6. 6.
    Patel SK, Lee J-K, Kalia VC (2017) Nanoparticles in biological hydrogen production: an overview. Indian J Microbiol.  https://doi.org/10.1007/s12088-017-0678-9 PubMedCentralGoogle Scholar
  7. 7.
    Otari S, Pawar S, Patel SK, Singh RK, Kim S-Y, Lee JH, Zhang L, Lee J-K (2017) Canna edulis leaf extract-mediated preparation of stabilized silver nanoparticles: characterization, antimicrobial activity, and toxicity studies. J Microbiol Biotechnol 27:731–738.  https://doi.org/10.4014/jmb.1610.10019 CrossRefPubMedGoogle Scholar
  8. 8.
    Singh R, Shedbalkar UU, Wadhwani SA, Chopade BA (2015) Bacteriagenic silver nanoparticles: synthesis, mechanism, and applications. Appl Microbiol Biotechnol 99:4579–4593.  https://doi.org/10.1007/s00253-015-6622-1 CrossRefPubMedGoogle Scholar
  9. 9.
    Gurunathan S (2014) Rapid biological synthesis of silver nanoparticles and their enhanced antibacterial effects against Escherichia fergusonii and Streptococcus mutans. Arab J Chem (in press)Google Scholar
  10. 10.
    Singh R, Wagh P, Bellare J (2013) Synthesis, optimization, and characterization of silver nanoparticles from Acinetobacter calcoaceticus and their enhanced antibacterial activity when combined with antibiotics. Int J Nanomed 8:4277–4290.  https://doi.org/10.2147/IJN.S48913 Google Scholar
  11. 11.
    Jain N, Bhargava A, Majumdar S, Tarafdar J, Panwar J (2011) Extracellular biosynthesis and characterization of silver nanoparticles using Aspergillus flavus njp08: a mechanism perspective. Nanoscale 3:635–641.  https://doi.org/10.1039/c0nr00656d CrossRefPubMedGoogle Scholar
  12. 12.
    Krishna IM, Reddy GB, Veerabhadram G, Madhusudhan A (2016) Eco-friendly green synthesis of silver nanoparticles using Salmalia malabarica: synthesis, characterization, antimicrobial, and catalytic activity studies. Appl Nano Sci 6:681–689.  https://doi.org/10.1007/s13204-015-0479-6 CrossRefGoogle Scholar
  13. 13.
    Park TJ, Lee SY, Heo NS, Seo TS (2010) In vivo synthesis of diverse metal nanoparticles by recombinant Escherichia coli. Angew Chem Int Ed 49:7019–7024.  https://doi.org/10.1002/anie.201001524 CrossRefGoogle Scholar
  14. 14.
    Peiris MK, Gunasekara CP, Jayaweera PM, Arachchi ND, Fernando N (2017) Biosynthesized silver nanoparticles: are they effective antimicrobials? Mem Inst Oswaldo Cruz 112:537–543.  https://doi.org/10.1590/0074-02760170023 CrossRefPubMedPubMedCentralGoogle Scholar
  15. 15.
    Rashid MU, Bhuiyan MKH, Quayum ME (2013) Synthesis of silver nano particles (Ag-NPs) and their uses for quantitative analysis of vitamin c tablets. Dhaka Univ J Pharm Sci 12:29–33.  https://doi.org/10.3329/dujps.v12i1.16297 CrossRefGoogle Scholar
  16. 16.
    Gurunathan S, Kalishwaralal K, Vaidyanathan R, Venkataraman D, Pandian SRK, Muniyandi J, Hariharan N, Eom SH (2009) Biosynthesis, purification and characterization of silver nanoparticles using Escherichia coli. Colloids Surf B 74:328–335.  https://doi.org/10.1016/j.colsurfb.2009.07.048 CrossRefGoogle Scholar
  17. 17.
    El-Shanshoury AE-RR, ElSilk SE, Ebeid ME (2011) Extracellular biosynthesis of silver nanoparticles using Escherichia coli ATCC 8739, Bacillus subtilis ATCC 6633, and Streptococcus thermophilus ESh1 and their antimicrobial activities. ISRN Nanotechnol 2011:1–7.  https://doi.org/10.5402/2011/385480 CrossRefGoogle Scholar
  18. 18.
    Jeevan P, Ramya K, Rena AE (2012) Extracellular biosynthesis of silver nanoparticles by culture supernatant of Pseudomonas aeruginosa. Indian J biotechnol 11:72–76 https://pdfs.semanticscholar.org/885a/b93862e3deb07611b4325c3234a7890e3b6d.pdf. Accessed 15 June 2016
  19. 19.
    Van Dong P, Ha CH, Binh LT, Kasbohm J (2012) Chemical synthesis and antibacterial activity of novel-shaped silver nanoparticles. Int Nano Lett 2:1–9.  https://doi.org/10.1186/2228-5326-2-9 CrossRefGoogle Scholar
  20. 20.
    Zielińska A, Skwarek E, Zaleska A, Gazda M, Hupka J (2009) Preparation of silver nanoparticles with controlled particle size. Procedia Chem 1:1560–1566.  https://doi.org/10.1016/j.proche.2009.11.004 CrossRefGoogle Scholar
  21. 21.
    Rout A, Jena PK, Sahoo D, Bindhani BK (2014) Green synthesis of silver nanoparticles of different shapes and its antibacterial activity against Escherichia coli. Int J Curr Microbiol App Sci 3:374–383 https://www.ijcmas.com/vol-3-4/Anandini%20Rout,%20et%20al.pdf. Accessed 15 Aug 2017
  22. 22.
    Logaranjan K, Raiza AJ, Gopinath SCB, Chen Y, Pandian K (2016) Shape- and size-controlled synthesis of silver nanoparticles using Aloe vera plant extract and their antimicrobial activity. Nanoscale Res Lett 11:1–9.  https://doi.org/10.1186/s11671-016-1725-x CrossRefGoogle Scholar
  23. 23.
    Sobczak-Kupiec A, Malina D, Wzorek Z, Zimowska M (2011) Influence of silver nitrate concentration on the properties of silver nanoparticles. IET Micro Nano Lett 6:656–660.  https://doi.org/10.1049/mnl.2011.0152 CrossRefGoogle Scholar
  24. 24.
    Sadowski Z, Maliszewska I, Grochowalska B, Polowczyk I, Kozlecki T (2008) Synthesis of silver nanoparticles using microorganisms. Mater Sci Poland 26:419–424 https://pdfs.semanticscholar.org/b2bc/87b05b455a7d00220465c6d231087e3fbfe4.pdf. Accessed 27 Aug 2017
  25. 25.
    Nanda A, Saravanan M (2009) Biosynthesis of silver nanoparticles from Staphylococcus aureus and its antimicrobial activity against MRSA and MRSE. Nanomedicine 5:452–456.  https://doi.org/10.1016/j.nano.2009.01.012 CrossRefPubMedGoogle Scholar
  26. 26.
    Kumar CG, Mamidyala SK (2011) Extracellular synthesis of silver nanoparticles using culture supernatant of Pseudomonas aeruginosa. Colloids Surf B 84:462–466.  https://doi.org/10.1016/j.colsurfb.2011.01.042 CrossRefGoogle Scholar
  27. 27.
    Paul D, Sinha SN (2014) Extracellular synthesis of silver nanoparticles using Pseudomonas aeruginosa KUPSB12 and its antibacterial activity. Jordan J of Biol Sci 7:245–250 http://jjbs.hu.edu.jo/files/v7n4/Paper%20Number%202m.pdf. Accessed 01 Sept 2017
  28. 28.
    Kushwaha A, Singh VK, Bhartariya J, Singh P, Yasmeen K (2015) Isolation and identification of E. coli bacteria for the synthesis of silver nanoparticles: characterization of the particles and study of antibacterial activity. Eur J Exp Biol 5:65–70 http://www.imedpub.com/articles/isolation-and-identification-of-ie-coliibacteria-for-the-synthesis-of-silver-nanoparticles-characterization-of-the-particles-and.pdf. Accessed 15 Sept 2017
  29. 29.
    Malarkodi C, Rajeshkumar S, Paulkumar K, Gnanajobitha G, Vanaja M, Annadurai G (2013) Bacterial synthesis of silver nanoparticles by using optimized biomass growth of Bacillus sp. Nanosci Nanotechnol 3:26–32 https://www.researchgate.net/profile/Rajeshkumar_Shanmugam/publication/236671464_Bacterial_synthesis_of_silver_nanoparticles_by_using_optimized_biomass_growth_of_Bacillus_sp/links/00b7d518d35968b7c9000000.pdf. Accessed 09 June 2017
  30. 30.
    Patil H, Borse S, Patil D, Patil U, Patil H (2011) Synthesis of silver nanoparticles by microbial method and their characterization. Arch Phys Res 2:153–158 https://www.researchgate.net/profile/Ulhas_Patil4/publication/268384142_Synthesis_of_Silver_Nanoparticles_by_Microbial_Method_and_Their_Characterization/links/54e337230cf2de71a71e58a0.pdf. Accessed 09 June 2017
  31. 31.
    Barapatre A, Aadil KR, Jha H (2016) Synergistic antibacterial and antibiofilm activity of silver nanoparticles biosynthesized by lignin-degrading fungus. Bioresour Bioprocess 3:8.  https://doi.org/10.1186/s40643-016-0083-y CrossRefGoogle Scholar
  32. 32.
    Phanjom P, Ahmed G (2015) Biosynthesis of silver nanoparticles by Aspergillus oryzae (MTCC no. 1846) and its characterizations. J Nanosci Nanotechnol 5:14–21.  https://doi.org/10.5923/j.nn.20150501.03 Google Scholar
  33. 33.
    Devika R, Elumalai S, Manikandan E, Eswaramoorthy D (2012) Biosynthesis of silver nanoparticles using the fungus Pleurotus ostreatus and their antibacterial activity. Sci Rep 1:1–5.  https://doi.org/10.4172/scientificreports.557 Google Scholar
  34. 34.
    Fayaz AM, Balaji K, Girilal M, Yadav R, Kalaichelvan PT, Venketesan R (2010) Biogenic synthesis of silver nanoparticles and their synergistic effect with antibiotics: a study against gram-positive and gram-negative bacteria. Nanomedicine 6:103–109.  https://doi.org/10.1016/j.nano.2009.04.006 CrossRefPubMedGoogle Scholar
  35. 35.
    Rajeshkumar S, Malarkodi C (2014) In vitro antibacterial activity and mechanism of silver nanoparticles against foodborne pathogens. Bioinorg Chem Appl 2014:1–10.  https://doi.org/10.1155/2014/581890 CrossRefGoogle Scholar
  36. 36.
    AbdelRahim K, Mahmoud SY, Ali AM, Almaary KS, Mustafa AE-ZM, Husseiny SM (2017) Extracellular biosynthesis of silver nanoparticles using Rhizopus stolonifer. Saudi J Biol Sci 24:208–216.  https://doi.org/10.1016/j.sjbs.2016.02.025 CrossRefPubMedGoogle Scholar
  37. 37.
    Pandey S, Thakur M, Shah R, Oza G, Mewada A, Sharon M (2013) A comparative study of economical separation and aggregation properties of biologically capped and thiol functionalized gold nanoparticles: selecting the eco-friendly trojan horses for biological applications. Colloids Surf B 109:25–31.  https://doi.org/10.1016/j.colsurfb.2013.03.011 CrossRefGoogle Scholar
  38. 38.
    Okafor F, Janen A, Kukhtareva T, Edwards V, Curley M (2013) Green synthesis of silver nanoparticles, their characterization, application and antibacterial activity. Int J Environ Res Publ health 10:5221–5238.  https://doi.org/10.3390/ijerph10105221 CrossRefGoogle Scholar
  39. 39.
    Cicek S, Gungor AA, Adiguzel A, Nadaroglu H (2015) Biochemical evaluation and green synthesis of nano silver using peroxidase from Euphorbia (Euphorbia amygdaloides) and its antibacterial activity. J Chem 2015:1–7.  https://doi.org/10.1155/2015/486948 (Hindawi online) CrossRefGoogle Scholar
  40. 40.
    Song H, Ko K, Oh I, Lee B (2006) Fabrication of silver nanoparticles and their antimicrobial mechanisms. Eur Cells Mater 11:58 http://thesilveredge.com/pdf/Fabrication%20of%Silver%20Nanoparticles%20and%20Their%20Antimicrobial%20Mechanisms.pdf. Accessed 15 September 2017
  41. 41.
    Long Y-M, Hu L-G, Yan X-T, Zhao X-C, Zhou Q-F, Cai Y, Jiang G-B (2017) Surface ligand controls silver ion release of nanosilver and its antibacterial activity against Escherichia coli. Int J Nanomedicine 12:3193.  https://doi.org/10.2147/IJN.S132327 CrossRefPubMedPubMedCentralGoogle Scholar
  42. 42.
    AshaRani P, Low Kah Mun G, Hande MP, Valiyaveettil S (2008) Cytotoxicity and genotoxicity of silver nanoparticles in human cells. ACS Nano 3:279–290.  https://doi.org/10.1021/nn800596w CrossRefGoogle Scholar
  43. 43.
    Liao J, Mo AC, Wu HK, Zhang JC, Li YB, Lv GY (2007) Antibacterial activity of silver-hydroxyapatite/titania nanoparticles on oral bacteria. Key Eng Mater 330–332:299–302.  https://doi.org/10.4028/www.scientific.net/KEM.330-332.299 CrossRefGoogle Scholar

Copyright information

© Association of Microbiologists of India 2018

Authors and Affiliations

  • M. M. K. Peiris
    • 1
  • S. S. N. Fernando
    • 1
  • P. M. Jayaweera
    • 2
  • N. D. H. Arachchi
    • 2
  • T. D. C. P. Guansekara
    • 1
  1. 1.Department of Microbiology, Faculty of Medical SciencesUniversity of Sri JayewardenepuraNugegodaSri Lanka
  2. 2.Department of Chemistry, Faculty of Applied SciencesUniversity of Sri JayewardenepuraNugegodaSri Lanka

Personalised recommendations