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Co-digestion of Biowastes to Enhance Biological Hydrogen Process by Defined Mixed Bacterial Cultures

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Abstract

Co-digestion of biowastes for hydrogen (H2) production using defined mixed cultures can overcome the high risk of failure due to contamination and imbalanced nutrient status. H2 production from biowastes—pea-shells, potato peels (PP), onion peels (OP) and apple pomace, either individually or in various combinations was evaluated by hydrolyzing with defined hydrolytic mixed bacterial culture (MHC5) and subjecting the hydrolysate to mixture of defined H2 producers (MMC6). Co-digestion of OP and PP hydrolysate supplemented at H2 production stage with GM-2 and M-9 media resulted in 95 and 102 l H2/kg of Total solids (TS), respectively compared to 84 l H2/kg of TS in control. Upscaling the process by digesting 4.0 l slurry (16-fold) resulted in 88.5 and 95 l H2/kg of TS, respectively compared to 72 l H2/kg of TS in control. Thus, H2 production by co-digestion of biowastes could be improved through the supplementation with very dilute medium (0.1 ×) and selection of suitable biowastes under unsterile conditions. The overall efficiency can be further enhanced by integrating it with bioprocesses for biopolymers such as polyhydroxyalkanoates and or biofuels like methane production.

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References

  1. Kumar P, Patel SKS, Lee JK, Kalia VC (2013) Extending the limits of Bacillus for novel biotechnological applications. Biotechnol Adv 31:1543–1561. https://doi.org/10.1016/j.biotechadv.2013.08.007

    Article  CAS  PubMed  Google Scholar 

  2. Patel SKS, Kumar P, Kalia VC (2012) Enhancing biological hydrogen production through complementary microbial metabolisms. Int J Hydrog Energy 37:10590–10603. https://doi.org/10.1016/j.ijhydene.2012.04.045

    Article  CAS  Google Scholar 

  3. Patel SKS, Selvaraj C, Mardina P, Jeong JH, Kalia VC, Kang YC, Lee JK (2016) Enhancement of methanol production from synthetic gas mixture by Methylosinus sporium through covalent immobilization. Appl Energy 171:383–391. https://doi.org/10.1016/j.apenergy.2016.03.022

    Article  CAS  Google Scholar 

  4. Sitthikitpanya S, Reungsang A, Prasertsan P (2018) Two-stage thermophilic bio-hydrogen and methane production from lime-pretreated oil palm trunk by simultaneous saccharification and fermentation. Int J Hydrog Energy 43:4284–4293. https://doi.org/10.1016/j.ijhydene.2018.01.063

    Article  CAS  Google Scholar 

  5. Venkata Mohan S, Nikhil GN, Chiranjeevi P, Reddy CN, Rohit MV, Naresh AK, Sankar O (2016) Waste biorefinery models towards sustainable circular bioeconomy: critical review and future perspectives. Bioresour Technol 215:2–12. https://doi.org/10.1016/j.biortech.2016.03.130

    Article  CAS  PubMed  Google Scholar 

  6. Ding L, Gutierrez EC, Cheng J, Xia A, O’Shea R, Guneratnam AJ, Murphy JD (2018) Assessment of continuous fermentative hydrogen and methane co-production using macro- and micro-algae with increasing organic loading rate. Energy 151:760–770. https://doi.org/10.1016/j.energy.2018.03.103

    Article  CAS  Google Scholar 

  7. Patel SKS, Kumar P, Singh S, Lee JK, Kalia VC (2015) Integrative approach to produce hydrogen and polyhydroxybutyrate from biowaste using defined bacterial cultures. Bioresour Technol 176:136–141. https://doi.org/10.1016/j.biortech.2014.11.029

    Article  CAS  PubMed  Google Scholar 

  8. Patel SKS, Mardina P, Kim D, Kim S-Y, Kalia VC, Kim I-W, Lee J-K (2016) Improvement in methanol production by regulating the composition of synthetic gas mixture and raw biogas. Bioresour Technol 218:202–208. https://doi.org/10.1016/j.biortech.2016.06.065

    Article  CAS  PubMed  Google Scholar 

  9. Patel SKS, Singh R, Kumar A, Jeong JH, Jeong SH, Kalia VC, Kim I-W, Lee J-K (2017) Biological methanol production by immobilized Methylocella tundrae using simulated biohythane as a feed. Bioresour Technol 241:922–927. https://doi.org/10.1016/j.biortech.2017.05.160

    Article  CAS  PubMed  Google Scholar 

  10. Kalia VC, Purohit HJ (2008) Microbial diversity and genomics in aid of bioenergy. J Ind Microbiol Biotechnol 35:403–419. https://doi.org/10.1007/s10295-007-0300-y

    Article  CAS  PubMed  Google Scholar 

  11. Patel SKS, Kalia VC (2013) Integrative biological hydrogen production: an overview. Indian J Microbiol 53:3–10. https://doi.org/10.1007/s12088-012-0287-6

    Article  CAS  PubMed  Google Scholar 

  12. Patel SKS, Lee JK, Kalia VC (2018) Beyond the theoretical yields of dark-fermentative biohydrogen. Indian J Microbiol 58:529–530. https://doi.org/10.1007/s12088-018-0759-4

    Article  CAS  PubMed  Google Scholar 

  13. Patel SKS, Purohit HJ, Kalia VC (2010) Dark fermentative hydrogen production by defined mixed microbial cultures immobilized on ligno-cellulosic waste materials. Int J Hydrog Energy 35:10674–10681. https://doi.org/10.1016/j.ijhydene.2010.03.025

    Article  CAS  Google Scholar 

  14. Patel SKS, Kumar P, Mehariya S, Purohit HJ, Lee JK, Kalia VC (2014) Enhancement in hydrogen production by co-cultures of Bacillus and Enterobacter. Int J Hydrog Energy 39:14663–14668. https://doi.org/10.1016/j.ijhydene.2014.07.084

    Article  CAS  Google Scholar 

  15. Kalia VC, Lal S, Ghai R, Mandal M, Chauhan A (2003) Mining genomic databases to identify novel hydrogen producers. Trends Biotechnol 21:152–156. https://doi.org/10.1016/S0167-7799(03)00028-3

    Article  CAS  PubMed  Google Scholar 

  16. Porwal S, Kumar T, Lal S, Rani A, Kumar S, Cheema S, Purohit HJ, Sharma R, Patel SKS, Kalia VC (2008) Hydrogen and polyhydroxybutyrate producing abilities of microbes from diverse habitats by dark fermentative process. Bioresour Technol 99:5444–5451. https://doi.org/10.1016/j.biortech.2007.11.011

    Article  CAS  PubMed  Google Scholar 

  17. Patel SKS, Singh M, Kalia VC (2011) Hydrogen and polyhydroxybutyrate producing abilities of Bacillus spp. from glucose in two stage system. Indian J Microbiol 51:418–423. https://doi.org/10.1007/s12088-011-0236-9

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  18. Patel SKS, Lee JK, Kalia VC (2018) Nanoparticles in biological hydrogen production: an overview. Indian J Microbiol 58:8–18. https://doi.org/10.1007/s12088-017-0678-9

    Article  CAS  PubMed  Google Scholar 

  19. Dinesh GK, Chauhan R, Chakma S (2018) Influence and strategies for enhanced biohydrogen production from food waste. Renew Sustain Energy Rev 92:807–822. https://doi.org/10.1016/j.rser.2018.05.009

    Article  CAS  Google Scholar 

  20. Kumari S, Das D (2016) Biologically pretreated sugarcane top as a potential raw material for the enhancement of gaseous energy recovery by two stage biohythane process. Bioresour Technol 218:1090–1097. https://doi.org/10.1016/j.biortech.2016.07.070

    Article  CAS  PubMed  Google Scholar 

  21. Patel SKS, Lee JK, Kalia VC (2016) Integrative approach for producing hydrogen and polyhydroxyalkanoate from mixed wastes of biological origin. Indian J Microbiol 56:293–300. https://doi.org/10.1007/s12088-016-0595-3

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  22. Patel SKS, Lee JK, Kalia VC (2017) Dark-fermentative biological hydrogen production from mixed biowastes using defined mixed cultures. Indian J Microbiol 57:171–176. https://doi.org/10.1007/s12088-017-0643-7

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  23. Prakash J, Sharma R, Patel SKS, Kim IW, Kalia VC (2018) Bio-hydrogen production by co-digestion of domestic wastewater and biodiesel industry effluent. PLoS ONE 13:e0199059. https://doi.org/10.1371/journal.pone.0199059

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  24. Wang J, Yin Y (2018) Fermentative hydrogen production using various biomass-based materials as feedstock. Renew Sustain Energy Rev 92:284–306. https://doi.org/10.1016/j.rser.2018.04.033

    Article  CAS  Google Scholar 

  25. Hassan SS, Williams GA, Jaiswal AK (2018) Emerging technologies for the pretreatment of lignocellulosic biomass. Bioresour Technol 262:310–318. https://doi.org/10.1016/j.biortech.2018.04.099

    Article  CAS  PubMed  Google Scholar 

  26. Patel SKS, Singh M, Kumar P, Purohit HJ, Kalia VC (2012) Exploitation of defined bacterial cultures for production of hydrogen and polyhydroxybutyrate from pea-shells. Biomass Bioenergy 36:218–225. https://doi.org/10.1016/j.biombioe.2011.10.027

    Article  CAS  Google Scholar 

  27. Singh G, Arya SK, Gupta V, Sharma P (2017) Enzyme technology for lignocellulosic biomass conversion and recycling to valuable paper and other products: challenges ahead. J Mol Biol Technol 2:105

    Google Scholar 

  28. Kumar P, Pant DC, Mehariya S, Sharma R, Kansal A, Kalia VC (2014) Ecobiotechnological strategy to enhance efficiency of bioconversion of wastes into hydrogen and methane. Indian J Microbiol 54:262–267. https://doi.org/10.1007/s12088-014-0467-7

    Article  PubMed  PubMed Central  Google Scholar 

  29. Singh M, Kumar P, Patel SKS, Kalia VC (2013) Production of polyhydroxyalkanoate co-polymer by Bacillus thuringiensis. Indian J Microbiol 53:77–83. https://doi.org/10.1007/s12088-012-0294-7

    Article  CAS  PubMed  Google Scholar 

  30. Kalia VC, Patel SKS, Kang YC, Lee JK (2019) Quorum sensing inhibitors as antipathogens: biotechnological applications. Biotechnol Adv 37:68–90. https://doi.org/10.1016/j.biotechadv.2018.11.006

    Article  CAS  PubMed  Google Scholar 

  31. Prakash J, Gupta RK, Priyanka XX, Kalia VC (2018) Bioprocessing of biodiesel industry effluent by immobilized bacteria to produce value-added products. Appl Biochem Biotechnol 185:179–190. https://doi.org/10.1007/s12010-017-2637-7

    Article  CAS  PubMed  Google Scholar 

  32. Mardina P, Li J, Patel SKS, Kim I-W, Lee J-K, Selvaraj C (2016) Potential of immobilized whole-cell Methylocella tundrae as a biocatalyst for methanol production from methane. J Microbiol Biotechnol 26:1234–1241. https://doi.org/10.4014/jmb.1602.02074

    Article  CAS  PubMed  Google Scholar 

  33. Patel SKS, Jeong J-H, Mehariya S, Otari SV, Madan B, Haw JR, Lee J-K, Zhang L, Kim I-W (2016) Production of methanol from methane by encapsulated Methylosinus sporium. J Microbiol Biotechnol 26:2098–2105. https://doi.org/10.4014/jmb.1608.08053

    Article  CAS  PubMed  Google Scholar 

  34. Patel SKS, Mardina P, Kim S-Y, Lee J-K, Kim I-W (2016) Biological methanol production by a type II methanotroph Methylocystis bryophila. J Microbiol Biotechnol 26:717–724. https://doi.org/10.4014/jmb.1601.01013

    Article  CAS  PubMed  Google Scholar 

  35. Patel SKS, Kumar P, Singh S, Lee JK, Kalia VC (2015) Integrative approach for hydrogen and polyhydroxybutyrate production. In: Kalia VC (ed) Microbial factories: waste treatment. Springer, New Delhi, pp 73–85. https://doi.org/10.1007/978-81-322-2598-0_5

    Chapter  Google Scholar 

  36. Patel SKS, Kondaveeti S, Otari SV, Pagolu RT, Jeong SH, Kim SC, Cho BK, Kang YC, Lee JK (2018) Repeated batch methanol production from a simulated biogas mixture using immobilized Methylocystis bryophila. Energy 145:477–485. https://doi.org/10.1016/j.energy.2017.12.142

    Article  CAS  Google Scholar 

  37. Patel SKS, Kumar V, Mardina P, Li J, Lestari R, Kalia VC, Lee J-K (2018) Methanol peoduction from simulated biogas mixtures by co-immobilized Methylomonas methanica and Methylocella tundrae. Bioresour Technol 263:25–32. https://doi.org/10.1016/j.biortech.2018.04.096

    Article  CAS  PubMed  Google Scholar 

  38. Singh M, Patel SKS, Kalia VC (2009) Bacillus subtilis as potential producer for polyhydroxyalkanoates. Microb Cell Fact 8:38. https://doi.org/10.1186/1475-2859-8-38

    Article  CAS  PubMed  PubMed Central  Google Scholar 

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Acknowledgements

This research was supported by Basic Science Research Program through the National Research Foundation of Korea (NRF) funded by the Ministry of Science, ICT and Future Planning (NRF-2018H1D3A2001746, 2013M3A6A8073184). This research was also supported by KU Research Professor Program of Konkuk University.

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Authors and Affiliations

  1. Department of Chemical Engineering, Konkuk University, 1 Hwayang-Dong, Gwangjin-Gu, Seoul, 05029, Republic of Korea

    Sanjay K. S. Patel, Jung-Kul Lee & Vipin Chandra Kalia

  2. Department of Microbial Biotechnology and Genomics, CSIR-Institute of Genomics and Integrative Biology, Mall Road, Delhi University Campus, Delhi, 110007, India

    Subhasree Ray & Jyotsana Prakash

  3. Department of Food Science and Biotechnology, Shin-Ansan University, Ansan, 15435, Republic of Korea

    Ji Hyang Wee & Sang-Yong Kim

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  1. Sanjay K. S. Patel
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Correspondence to Jung-Kul Lee or Vipin Chandra Kalia.

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Patel, S.K.S., Ray, S., Prakash, J. et al. Co-digestion of Biowastes to Enhance Biological Hydrogen Process by Defined Mixed Bacterial Cultures. Indian J Microbiol 59, 154–160 (2019). https://doi.org/10.1007/s12088-018-00777-8

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