Annotation and De Novo Sequence Characterization of Extracellular β-Fructofuranosidase from Penicillium chrysogenum Strain HKF42
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The genome of a fungal strain Penicillium chrysogenum strain HKF42, which can grow on 20% sucrose has been annotated for 7595 protein coding sequences. On mining of CAZymes, we could annotate a β-fructofuranosidase gene responsible for fructo-oligosaccharides (FOS) synthesis which is a known prebiotic. The enzyme activity was demonstrated and validated with the generation of FOS as kestose and nystose.
KeywordsPrebiotic Fructo-oligosaccharides Penicillium β-Fructofuranosidase
Authors would like to acknowledge to CSIR-NEERI for providing essential resources for the research work [KRC No. CSIR-NEERI/KRC/2017/SEP/EBGD/2]. Vaibhav Gujar is thankful to University Grants Commission (UGC), New Delhi for providing Junior and Senior Research Fellowship for carrying out this research.
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