Abstract
In this study, we present a microarray approach for the typing of influenza A and B viruses, and the subtyping of H1 and H3 subtypes. We designed four pairs of specific multiplex RT-PCR primers and eight specific oligonucleotide probes and prepared microarrays to identify the specific subtype of influenza virus. Through amplification and fluorescent marking of the multiplex RT-PCR products on the M gene of influenza A and B viruses and the HA gene of subtypes H1 and H3, the PCR products were hybridized with the microarray, and the results were analyzed using a microarray scanner. The results demonstrate that the chip developed by our research institute can detect influenza A and B viruses specifically and identify the subtypes H1 and H3 at a minimum concentration of 1 × 102 copies/μL of viral RNA. We tested 35 clinical samples and our results were identical to other fluorescent methods. The microarray approach developed in this study provides a reliable method for the monitoring and testing of seasonal influenza.
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The work was supported by the National Key Technology Research and Development Program of the Ministry of Science and Technology of China (Grant No. 2010BAD04B02).
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The authors declared that they have no conflicts of interest.
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Mingyao Tian, Yufei Tian and Yang Li contributed equally to this work.
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Tian, M., Tian, Y., Li, Y. et al. Microarray Multiplex Assay for the Simultaneous Detection and Discrimination of Influenza A and Influenza B Viruses. Indian J Microbiol 54, 211–217 (2014). https://doi.org/10.1007/s12088-013-0432-x
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DOI: https://doi.org/10.1007/s12088-013-0432-x