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Optimization of Process Variables for Lipase Biosynthesis from Rhizopus oligosporus NRRL 5905 Using Evolutionary Operation Factorial Design Technique

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Abstract

Extracellular lipase was produced from Rhizopus oligosporus NRRL 5905 through solid state fermentation (SSF). To provide an optimum fermentation conditions for maximum lipase yield, five process variables (temperature, liquid–solid ratio, pH, incubation period and spore concentration) were optimized using evolutionary operation (EVOP) factorial design technique taking into account the interaction between the process variables. Optimization through EVOP resulted in around 3 fold increase in lipase activity (77 U gds−1) at a liquid–solid ratio of 1.5:1, fermentation temperature of 35°C, initial fermentation pH 6, incubation period 5 days and a spore concentration of 108 spores ml−1.

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Acknowledgements

The authors wish to acknowledge Department of Biotechnology and Council of Scientific and Industrial Research for providing financial support to carry out this work.

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Authors and Affiliations

  1. Microbial Biotechnology and Downstream Processing Laboratory, Department of Agricultural and Food Engineering, Indian Institute of Technology, Kharagpur, West Bengal, 721302, India

    Paramita Mahapatra, Annapurna Kumari, Vijay Kumar Garlapati & Rintu Banerjee

  2. Department of Chemistry, Indian Institute of Technology, Kharagpur, West Bengal, 721302, India

    A. Nag

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  1. Paramita Mahapatra
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  2. Annapurna Kumari
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  3. Vijay Kumar Garlapati
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  4. Rintu Banerjee
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  5. A. Nag
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Correspondence to Rintu Banerjee.

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Mahapatra, P., Kumari, A., Garlapati, V. et al. Optimization of Process Variables for Lipase Biosynthesis from Rhizopus oligosporus NRRL 5905 Using Evolutionary Operation Factorial Design Technique. Indian J Microbiol 50, 396–403 (2010). https://doi.org/10.1007/s12088-011-0071-z

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  • DOI: https://doi.org/10.1007/s12088-011-0071-z

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