Journal of Cell Communication and Signaling

, Volume 12, Issue 1, pp 265–270 | Cite as

Characterization of bone morphology in CCN5/WISP5 knockout mice

Research Article


CCN5/WISP2 is part of the CCN family of matricellular proteins, but is distinct in that it lacks the C-terminal (CT) domain. Although CCN5 has been shown to impact cell proliferation and differentiation in vitro, its role in vivo is unclear. We therefore generated mice using ES cells developed by the Knockout Mouse Project (KOMP) in which exons 2-5, which encode the all of the conserved protein coding regions, are replaced by a lacZ cassette. Ccn5 LacZ/LacZ mice were viable and apparently normal. Based on previous studies showing that CCN5 impacts osteoblast proliferation and differentiation, we performed an analysis of adult bone phenotype. LacZ expression was examined in adult bone, and was found to be strong within the periosteum, but not in trabecular bone or bone marrow. Micro-CT analysis revealed no apparent changes in bone mineral density (BMD) or bone tissue volume (BV/TV) in Ccn5 LacZ/LacZ mice. These studies indicate that CCN5 is not required for normal bone formation, but they do not rule out a role in mechanotransduction or repair processes. The availability of Ccn5 LacZ mice enables studies of CCN5 expression and function in multiple tissues.


CCN Bone 



This work was supported by NIAMS/NIH grants R01 AR052686 and R21 AR071734 to KML.


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Copyright information

© The International CCN Society 2018

Authors and Affiliations

  1. 1.Department of PediatricsRush University Medical CenterChicagoUSA
  2. 2.Department of Pathology and Lab Medicine, David Geffen School of MedicineUniversity of California, Los AngelesLos AngelesUSA
  3. 3.Department of Orthopaedic Surgery, David Geffen School of MedicineUniversity of California, Los AngelesLos AngelesUSA
  4. 4.Department of Molecular, Cell and Developmental BiologyUniversity of California, Los AngelesLos AngelesUSA

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