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Detection of Epstein-Barr and Human Papilloma Viruses in the Middle Ear Squamous Cell Carcinoma

  • Agus Surono
  • Bambang Hariwiyanto
  • Edhie Samodra
Original Article
  • 129 Downloads

Abstract

The uncommon ear tumor of middle ear squamous cell carcinoma (MESCC) is thought to be associated with the history of long-term chronic otitis media in the most cases. The main etiologic factor of MESCC is still unclear and may be multifactorial. Infections of Epstein-Barr virus (EBV) and Human Papillomavirus (HPV) are considered as one of the etiologic factor of MESCC. Previous studies have shown that the EBV and HPV have been detected in MESCC. Although the EBV and HPV have been implicated in human malignancies, their roles in pathogenesis of MESCC have not been elucidated. There has never been report on the presence of EBV and HPV in Indonesian MESCC. This study aimed to determine the presence of EBV and HPV in MESCC. Seven paraffin-embedded tissues of speciment from biopsy were analyzed for the presence of EBV and HPV by immunohistochemistry, stained using polyclonal antibody anti EBNA1 and anti HPV. The samples consisted of 4 (57 %) males and 3 (43 %) females with age range of 26–87 years old. Immunohistochemistry result demonstrated that EBV was detected in three of seven (43 %) and HPV in two of seven (29 %) samples. Coexistence of the presence of EBV and HPV were found in one of seven (14 %) sample. The presence of EBV and HPV in MESCC suggests that viral infection may play an important etiologic role in the carcinogenesis of middle ear.

Keywords

Middle ear squamous cell carcinoma EBV HPV Immunohistochemistry 

Notes

Acknowledgments

The authors would thank to Hariyadi MD for confirming the immunohisto-chemistry results, Arif Budiyanto MD for revising the manuscript, Mrs Agustin for preparing materials and laboratory work, and Ms Yuni as the data manager in Oncology division, Department of Otolaryngology Head and Neck Surgery Faculty of Medicine UGM. This study was supported by Dana Masyarakat FK UGM.

Funding

This work was supported by grant of Dana Masyarakat A21/2012 (Dr. Surono) from Faculty of Medicine, Universitas Gadjah Mada, Yogyakarta, Indonesia.

Complaisance with Ethical Standards

Conflict of interest

The authors confirm that they have no potential conflicts of interests that could inappropriately influence or bias this work.

Ethical Approval

This article does not contain any studies with human participants or animals performed by any of the authors.

References

  1. 1.
    Shibuya K, Mather CD, Boschi-Pinto C, Lopez AD, Murray CJ (2002) Global and regional estimates of cancer mortality and incidency by site: II. Results for the global burden of disease 2002. BMC Cancer 2:37CrossRefPubMedPubMedCentralGoogle Scholar
  2. 2.
    Gasco M, Crook T (2003) The p53 network in head and neck cancer. Oral Oncol 39:222–2231CrossRefPubMedGoogle Scholar
  3. 3.
    McKaig RG, Baric RS, Olshan AF (1998) Human papillomavirus and head and neck cancer: epidemiology and molecular biology. Head Neck 20:250–265CrossRefPubMedGoogle Scholar
  4. 4.
    Szekely L, Selivanoca G, Magnusson KP, Klein G, Wiman KG (1993) EBNA-5, and Epstein-Barr virus-encoded nuclear antigen, binds to the retinoblastoma and p53 protein. Proc Natl Acad Sci USA 90:5455–5459CrossRefPubMedPubMedCentralGoogle Scholar
  5. 5.
    Syrjänen S, Laine P, Niemla M, Happonen RP (1989) Oral hairy leukoplakia is not specific sign of HIV infection but related to immunosuppression in general. J Oral Pathol Med 18:28–31CrossRefPubMedGoogle Scholar
  6. 6.
    Bernard HU, Chan SY, Manos MM, Ong CK, Villa LL, Delius H, Peyton CL, Bauer HM, Wheeler CM (1994) Identification and assessment of known and novel human papillomavirus by polymerase chain reaction, restriction digest fingerprinting, nucleotide sequence and phylogenetic algorithms. J Infect Dis 170:1077–1085CrossRefPubMedGoogle Scholar
  7. 7.
    Chang F, Syrjänen S, Nuutinen J, Karja J, Syrjänen K (1990) Detection of human papillomavirus (HPV) DNA in oral squamous cell carcinomas by in situ hybridization and polymerase chain reaction. Arch Dermatol Res 282:493–497CrossRefPubMedGoogle Scholar
  8. 8.
    Politzer A (1883) Textbook of diseases of the ear (trans: Cassells HC). Lea & Son, Philadelphia, pp 729–734Google Scholar
  9. 9.
    Lewis JS (1975) Temporal bone resection: review of 100 cases. Arch Otolaryngol 101:23–25CrossRefPubMedGoogle Scholar
  10. 10.
    Morton RP, Stell PM, Derrick PO (1984) Epidemiology of cancer of the middle ear cleft. Cancer 53:1612–1617CrossRefPubMedGoogle Scholar
  11. 11.
    Kenyon G, Marks P, Scholtz C, Dhillon R (1985) Squamous cell carcinoma of the middle ear: a 25-year retrospective study. Ann Otol Rhinol Laryngol 94:273–277PubMedGoogle Scholar
  12. 12.
    Jin YT, Tsai ST, Li C, Chang KC, Yan JJ, Chao WY, Eng HL, Chou TY, Wu TC, Su IJ (1997) Prevalence of human papillomavirus in middle ear carcinoma associated with chronic otitis media. Am J Pathol 150:1327–1333PubMedPubMedCentralGoogle Scholar
  13. 13.
    Savić DLJ, Djerić DR (1991) Malignant tumours of the middle ear. Clin Otolaryngol Allied Sci 16:87–89CrossRefPubMedGoogle Scholar
  14. 14.
    Michaels L, Wells M (1980) Squamous carcinoma of the middle ear. Clin Otolaryngol Allied Sci 5:235–248CrossRefPubMedGoogle Scholar
  15. 15.
    Bergmann K, Hoppe F, He Y, Helms J, Mϋller-Hermelink HK, Stremlau A, de Villiers EM (1994) Human papillomavirus DNA in cholesteatomas. Int J Cancer 59:463–466CrossRefPubMedGoogle Scholar
  16. 16.
    Ruben RJ, Thaler SU, Holzer N (1977) Radiation induced carcinoma of the temporal bone. Laryngoscope 87:1613–1621CrossRefPubMedGoogle Scholar
  17. 17.
    Tsai ST, Li C, Jin YT, Chao WY, Su IJ (1997) High prevalence of human papilloma virus types 16 and 18 in middle-ear carcinomas. Int J Cancer 71:208–212CrossRefPubMedGoogle Scholar
  18. 18.
    Leung SY, Yuen ST, Ho CM, Kwong WK, Chung LP (1998) Presence of Epstein-Barr virus in lymphoepithelioma-like carcinoma of the middle ear. J Clin Pathol 51:602–605CrossRefPubMedPubMedCentralGoogle Scholar
  19. 19.
    Austin JR, Stewart KL, Fawzi N (1994) Squamous cell carcinoma of the external auditory canal: therapeutic prognosis based on a proposed staging system. Arch Otolaryngol Head Neck Surg 120:1228–1232CrossRefPubMedGoogle Scholar
  20. 20.
    Sasaki CT (2001) Distant metastases from ear and temporal bone cancer. ORL 63:250–251CrossRefPubMedGoogle Scholar
  21. 21.
    Khanna R, Burrows SR, Moss DJ (1995) Immune regulation in Epstein-Barr virus associated disease. Microbiol Rev 59:387–405PubMedPubMedCentralGoogle Scholar
  22. 22.
    Kempkes B, Strobl LJ, Bornkamm GW, Zimer-Strobl U (2005) EBNA-2 and notch signaling. In: Robertson EE (ed) Epstein-Barr virus, Chapter 23. Caister Academic Press, Norfolk, pp 463–499Google Scholar
  23. 23.
    Leung SY, Chung LP, Yuen ST, Wong MP, Kwong WK (1995) Lymphoepithelial carcinoma of the salivary gland: in situ detection of Epstein-Barr virus. J Clin Pathol 48:1022–1027CrossRefPubMedPubMedCentralGoogle Scholar
  24. 24.
    Leung SY, Yuen ST, Chung LP, Keong WK, Wong MP, Chan SY (1995) Epstein-Barr virus is present in a wide histological spectrum of sinonasal carcinomas. Am J Surg Pathol 19:994–1001CrossRefPubMedGoogle Scholar
  25. 25.
    Lopategui JR, Gaffey MJ, Frierson HF, Chan JK, Mills SE, Chang KL, Chen YY, Weiss LM (1994) Detection of Epstein-Barr viral RNA in sinonasal undifferentiated carcinoma from Western and Asian patients. Am J Surg Pathol 18:391–398CrossRefPubMedGoogle Scholar
  26. 26.
    Nicholls JM, Pittaluga S, Chung LP, So KC (1994) The association between carcinoma of the tonsil and Epstein-Barr virus: a study using radiolabelled in situ hybridization. Pathology 26:94–98CrossRefPubMedGoogle Scholar
  27. 27.
    Leung SY, Chung LP, Ho CM, Yuen ST, Wong MP, Kwong WK (1996) An Epstein-Barr virus positive undifferentiated carcinoma in the lacrimal sac. Histopathology 28:71–75CrossRefPubMedGoogle Scholar
  28. 28.
    Dimery IW, Lee JS, Blick M, Pearson G, Spitzer G, Hong WK (1988) Association of the Epstein-Barr virus with lymphoepithelioma of the thymus. Cancer 61:2475–2480CrossRefPubMedGoogle Scholar
  29. 29.
    Butler AE, Colby TV, Weiss L, Lombard C (1989) Lymphoepithelioma-like carcinoma of the lung. Am J Surg Pathol 13:632–639CrossRefPubMedGoogle Scholar
  30. 30.
    zur Hausen H, de Villiers EM (1994) Human papillomaviruses. Annu Rev Microbiol 48:427–447CrossRefPubMedGoogle Scholar
  31. 31.
    Tase T, Okagaki T, Clark BA, Manias DA, Ostrow RS, Twiggs LB, Faras AJ (1988) Human papillomavirus types and localization in adenocarcinoma and adenosquamous carcinoma of the uterine cervix: a study by in situ DNA hybridization. Cancer Res 48:993–998PubMedGoogle Scholar
  32. 32.
    Stoler MH, Mills SE, Gersell DJ, Walker AN (1991) Small-cell neuroendocrine carcinoma of the cervix. A human papillomavirus type 18-associated cancer. Am J Surg Pathol 15:28–32CrossRefPubMedGoogle Scholar
  33. 33.
    Matlashewski G (1989) The cell biology of human papillomavirus transformed cells. Anticancer Res 9:1447–1456PubMedGoogle Scholar
  34. 34.
    Howley PM (1991) Role of the human papillomaviruses in human cancer. Cancer Res 51(18 Suppl):5019s–5022sPubMedGoogle Scholar
  35. 35.
    Chen B, Yin H, Dhurandhar N (1994) Detection of human papillomavirus DNA in esophageal squamous cell carcinomas by the polymerase chain reaction using general consensus primers. Hum Pathol 25:920–923CrossRefPubMedGoogle Scholar

Copyright information

© Association of Otolaryngologists of India 2016

Authors and Affiliations

  1. 1.Department of Otorhinolaryngology-Head and Neck Surgery, Faculty of MedicineUniversitas Gadjah MadaYogyakartaIndonesia

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