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Pramana

, Volume 71, Issue 2, pp 359–368 | Cite as

Effect of macromolecular crowding on the rate of diffusion-limited enzymatic reaction

  • Manish Agrawal
  • S. B. SantraEmail author
  • Rajat Anand
  • Rajaram Swaminathan
Article

Abstract

The cytoplasm of a living cell is crowded with several macromolecules of different shapes and sizes. Molecular diffusion in such a medium becomes anomalous due to the presence of macromolecules and diffusivity is expected to decrease with increase in macromolecular crowding. Moreover, many cellular processes are dependent on molecular diffusion in the cell cytosol. The enzymatic reaction rate has been shown to be affected by the presence of such macromolecules. A simple numerical model is proposed here based on percolation and diffusion in disordered systems to study the effect of macromolecular crowding on the enzymatic reaction rates. The model qualitatively explains some of the experimental observations.

Keywords

Enzyme kinetics Monte Carlo percolation random walk obstacle 

PACS Nos

02.50.Ey 05.40.Jc 05.60.Cd 

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Copyright information

© Indian Academy of Sciences 2008

Authors and Affiliations

  • Manish Agrawal
    • 1
  • S. B. Santra
    • 2
    Email author
  • Rajat Anand
    • 1
  • Rajaram Swaminathan
    • 1
  1. 1.Department of BiotechnologyIndian Institute of Technology GuwahatiGuwahatiIndia
  2. 2.Department of PhysicsIndian Institute of Technology GuwahatiGuwahatiIndia

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