Mapping QTL controlling agronomic traits in a doubled haploid population of winter oilseed rape (Brassica napus L.)
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Identification of superior alleles for agronomic traits in genetic resources of oilseed rape (Brassica napus L.) would be useful for improving the performance of locally adapted cultivars in Iran. The objective of the present work was to analyse the genetic variation and inheritance of important agronomic traits in a doubled haploid population derived from a cross between two German oilseed rape cultivars, Sansibar and Oase. Field experiments were performed in 2016–2017 with 200 doubled haploid lines and the parental genotypes applying an alpha-lattice design with two replicates. Phenological traits were recorded during the cultivation period and at maturity, seed yield, yield components and seed quality traits were determined. Significant genetic variation was found in most of the traits and heritabilities ranged from medium (48.5%) for days to end of flowering to high (92.6%) for oil content. A molecular marker linkage map was used to map 36 QTL for different traits on 17 linkage groups. Between three and four QTL were identified for each seed yield, seed weight, oil and protein content. Some of the plant material and positive QTL alleles identified for agronomic traits may be useful for improving those characters in locally adapted cultivars in Iran.
Keywordsgenetic resources oil content QTL mapping yield yield components
- Abd El-Mohsen A. A. and Abo-Hegazy S. R. 2013 Comparing the relative efficiency of two experimental designs in wheat field trials. Sci. Res. Rev. J. 1, 101–109.Google Scholar
- Faraji F. 2013 The role of yield components to determine seed yield of canola (Brassica napus L.) in Gonbad area. J. Plant. Product. 20, 217–234 (In Persian).Google Scholar
- Geng X., Jiang C., Yang J., Wang L., Wu X. and Wei W. 2016 Rapid identification of candidate genes for seed weight using the SLAF-Seq method in Brassica napus. PLoS One , https://doi.org/10.1371/journal.pone.0147580.
- Kimber D. and Mcgregor D. I. 1995 Brassica oilseeds-production and utilization. CAB. Inter. Wallingford, UK.Google Scholar
- Liu H., Du D., Guo S., Xiao L., Zhao Z., Zhao Z. et al. 2016b QTL analysis and the development of closely linked markers for days to flowering in spring oilseed rape (Brassica napus L.). Mol. Breed. 36, 52.Google Scholar
- Rezaizad A., Mohammadi V. A., Zali A., Zeinali H., Mardi M. and Delourme R. 2010 Mapping QTLs for some agronomic traits in rapeseed (Brassica napus L.). Iranian. J. Field. Crop. Sci. 40, 133–142 (In Persian).Google Scholar
- Rezaizad A., Mohammadi V. A., Zali A., Zeinali H. and Mardi M. 2012 Study of important agronomic traits and their relationships under normal and drought stress conditions in doubled haploid lines of oilseed rape. Iranian. J. Field. Crop. Sci. 42, 683–694 (In Persian).Google Scholar
- Shufen Z., Tingdong F., Jiacheng Z., Jianping W., Yancheng W., Chaozhi M. et al. 2007 QTL mapping and epistasis analysis for plant height and height to the first branch in rapeseed (Brassica napus L.). BIOTECHNOLOGY: Gene Clon. Func. Anal. IRC Proceedings, 12th IRC Wuhan China, 1, 232–235.Google Scholar
- Tunçtürk M. and Çiftçi V. 2007 Relationships between yield and some yield components in rapeseed (Brassica napus ssp. oleifera L.) cultivars by using correlations and path analysis. Pak. J. Bot. 39, 81–84.Google Scholar
- Wang S., Basten C. J. and Zeng Z. B. 2012 Windows QTL cartographer 2.5. Department of Statistics, North Carolina State University, Raleigh, NC.Google Scholar
- Weir B. 2008 The Biology of Brassica napus L. (canola). Department of Health and Aging Office of the Gene Technology Regulator. Australian Governmment.Google Scholar
- Zheng M., Peng C., Liu H., Min Tang M., Yang H., Li X. et al. 2017 Genome- wide association study reveals candidate genes for control of plant height, branch initiation height and branch number in rapeseed (Brassica napus L.) Front. Plant. Sci. 8, 1246.Google Scholar
- Zhou L., Li Y., Hussain N., Li Z., Wu D. and Jiang L. 2016 Allelic variation of BnaC.TT2.a and its association with seed coat color and fatty acids in rapeseed (Brassica napus L.). PLoS One , https://doi.org/10.1371/journal.pone.0146661.