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Journal of Genetics

, Volume 97, Issue 5, pp 1353–1361 | Cite as

Characterization of a splice variant of soybean ERECTA devoid of an intracellular kinase domain in response to shade stress

  • Junbo DuEmail author
  • Yan Li
  • Xin Sun
  • Liang Yu
  • Hengke Jiang
  • Qiulin Cao
  • Jing Shang
  • Mengyuan Sun
  • Yi Liu
  • Kai Shu
  • Jiang Liu
  • Taiwen Yong
  • Weiguo Liu
  • Feng Yang
  • Xiaochun Wang
  • Chunyan Liu
  • Wenyu YangEmail author
Research Article
  • 90 Downloads

Abstract

The receptor-like kinase ERECTA (ER) plays vital roles in plant adaptation under environmental stress including shade avoidance in Arabidopsis. In our previous study, we identified four ER paralogues in soybean (GmERs) that showed high similarities to Arabidopsis ER. Each GmER was predicted to generate diverse alternative splicing variants. However, whether soybean GmERs contribute to shade avoidance is unknown. Here we report our characterization of GmERs in response to shading. Promoter::GUS staining analysis shows that expression of GmER paralogous genes was differentially induced under shade stress. Further analyses show that GmERa.1 and GmERa.2 exhibit a larger distinction in length than the other GmER variants. GmERa.2 has the shortest length of amino acid with only 15 leucine-rich repeats which is the part of the extracellular domain of GmERa.1. Overexpression of GmERa.2 fully rescued the hypocotyl length, leaf area and petiole length, and the sensitivity of the hypocotyl of Arabidopsis mutant er-3 to shading, suggesting that the truncated extracellular domain of GmERa might contribute importantly to shade avoidance.

Keywords

ERECTA extracellular domain receptor-like kinase shade avoidance soybean 

Notes

Acknowledgements

This work was supported by the National Natural Science Foundation of China grants 31401308, 31671445 and 31371555, and Sichuan Science and Technology Programme (2018HH0108). We are grateful to Muhammad Ahsan Asghar for reviewing the English grammar of the manuscript and to Arabidopsis Biological Resource Center for providing some of the T-DNA insertional Arabidopsis mutant seeds.

Supplementary material

12041_2018_1035_MOESM1_ESM.docx (16 kb)
Supplementary material 1 (docx 15 KB)
12041_2018_1035_MOESM2_ESM.pdf (150 kb)
Supplementary material 2 (pdf 150 KB)
12041_2018_1035_MOESM3_ESM.tif (268 kb)
Supplementary material 3 (tif 268 KB)

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Copyright information

© Indian Academy of Sciences 2018

Authors and Affiliations

  • Junbo Du
    • 1
    Email author
  • Yan Li
    • 1
  • Xin Sun
    • 1
  • Liang Yu
    • 1
  • Hengke Jiang
    • 1
  • Qiulin Cao
    • 1
  • Jing Shang
    • 1
  • Mengyuan Sun
    • 1
  • Yi Liu
    • 1
  • Kai Shu
    • 1
  • Jiang Liu
    • 1
  • Taiwen Yong
    • 1
  • Weiguo Liu
    • 1
  • Feng Yang
    • 1
  • Xiaochun Wang
    • 1
  • Chunyan Liu
    • 1
  • Wenyu Yang
    • 1
    Email author
  1. 1.College of Agronomy, Sichuan Agricultural University/Sichuan Engineering Research Center for Crop Strip Intercropping system/Key Laboratory of Crop Eco-physiology and Farming System in Southwest China (Ministry of Agriculture)Sichuan Agricultural UniversityChengduPeople’s Republic of China

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