Characterization of a splice variant of soybean ERECTA devoid of an intracellular kinase domain in response to shade stress
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The receptor-like kinase ERECTA (ER) plays vital roles in plant adaptation under environmental stress including shade avoidance in Arabidopsis. In our previous study, we identified four ER paralogues in soybean (GmERs) that showed high similarities to Arabidopsis ER. Each GmER was predicted to generate diverse alternative splicing variants. However, whether soybean GmERs contribute to shade avoidance is unknown. Here we report our characterization of GmERs in response to shading. Promoter::GUS staining analysis shows that expression of GmER paralogous genes was differentially induced under shade stress. Further analyses show that GmERa.1 and GmERa.2 exhibit a larger distinction in length than the other GmER variants. GmERa.2 has the shortest length of amino acid with only 15 leucine-rich repeats which is the part of the extracellular domain of GmERa.1. Overexpression of GmERa.2 fully rescued the hypocotyl length, leaf area and petiole length, and the sensitivity of the hypocotyl of Arabidopsis mutant er-3 to shading, suggesting that the truncated extracellular domain of GmERa might contribute importantly to shade avoidance.
KeywordsERECTA extracellular domain receptor-like kinase shade avoidance soybean
This work was supported by the National Natural Science Foundation of China grants 31401308, 31671445 and 31371555, and Sichuan Science and Technology Programme (2018HH0108). We are grateful to Muhammad Ahsan Asghar for reviewing the English grammar of the manuscript and to Arabidopsis Biological Resource Center for providing some of the T-DNA insertional Arabidopsis mutant seeds.
- Du J., Sun X., Sun M., Jiang H., Li Y., Wan C. et al. 2017b Identification and expression analysis of ERECTA homologous genes in Glycine max. Int. J. Agric. Biol. 19, 1497–1504.Google Scholar
- Rédei G. P., Koncz C., Chua N. H. and Schell J. 1992 A heuristic glance at the past of Arabidopsis genetics. In Methods in Arabidopsis research (ed. C. Koncz, N. H. Chua and J. Schell), pp. 1–15. World Scientific, Singapore.Google Scholar
- Shiu S. H. and Bleecker A. B. 2001 Plant receptor-like kinase gene family: diversity, function, and signaling. Science’s STKE 2001, re22–re22.Google Scholar