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Journal of Genetics

, Volume 97, Issue 5, pp 1289–1294 | Cite as

Expression of mitofusin 2 in placentae of women with gestational diabetes mellitus

  • Bingjin Chen
  • Yajuan GeEmail author
  • Honglin Wang
  • Haihong Zhu
  • Jinyu Xu
  • Zhenghong Wu
  • Siye Tang
Research Article
  • 55 Downloads

Abstract

Gestational diabetes mellitus (GDM) represents a common carbohydrate metabolism disorder during pregnancy. The objective of this study was to evaluate the expression levels of mitofusin 2 (MFN2) expression in placentae of GDM patients compared to that in the placental tissues from normal uncomplicated pregnancies. A total of 70 subjects were enrolled from September 2014 to June 2016, including 42 patients with GDM (the GDM group) and 28 normal uncomplicated pregnancies (the control group). Immunohistochemical staining and qRT-PCR were used for the detection of the expression levels and distribution of MFN2 in the placentae of GDM patients and normal controls. Kolmogorov–Smirnov test was used for statistical analysis. \(P<0.05\) and \(P<0.01\) were used for assessing statistical significance. The baseline characteristics were comparable in both groups. The 1-h and 2-h postprandial glucose levels (PPG) were \(7.94\pm 1.26\) versus \(6.88\pm 0.51\) mmol/L and \(7.01\pm 1.34\) versus \(6.14\pm 0.63\) mmol/L, respectively, for the GDM group and the control group (\(P<0.05\)). The relative expression levels of MFN2 mRNA were \(0.982\pm 1.242\) for GDM and \(1.257\pm 0.815\) for control, respectively, with significant between group difference (\(P<0.01\)). Immunohistochemical staining analysis showed that MFN2 was mostly distributed in the cytoplasm of syncytiotrophoblasts under optical microscopy. Additionally, about 50% of samples of the GDM group were within the intensity of moderate staining of MFN2 and more than 50% of patients in the control group were within the intensity of strong staining of MFN2. The expression levels of MFN2 in GDM placentae was significantly lower compared to that of placentae from normal uncomplicated pregnancies.

Keywords

gestational diabetes mellitus placenta mitofusin 2 immunohistochemical staining 

Notes

Acknowledgements

This work was supported by the Minhang District Natural Science Foundation of Shanghai (programme no. 2015MHZ014).

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Copyright information

© Indian Academy of Sciences 2018

Authors and Affiliations

  • Bingjin Chen
    • 1
  • Yajuan Ge
    • 2
    Email author
  • Honglin Wang
    • 1
  • Haihong Zhu
    • 3
  • Jinyu Xu
    • 4
  • Zhenghong Wu
    • 1
  • Siye Tang
    • 2
  1. 1.Department of Obstetrics and GynecologyShanghai Fifth People’s Hospital of Fudan UniversityShanghaiPeople’s Republic of China
  2. 2.Department of Clinical Laboratory MedicineShanghai Fifth People’s Hospital of Fudan UniversityShanghaiPeople’s Republic of China
  3. 3.Department of ObstetricsShanghai Baijia Maternity HospitalShanghaiPeople’s Republic of China
  4. 4.Department of OutpatientShanghai Fifth People’s Hospital of Fudan UniversityShanghaiPeople’s Republic of China

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