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Journal of Genetics

, Volume 97, Issue 5, pp 1379–1388 | Cite as

The jasmonate-responsive transcription factor CbWRKY24 regulates terpenoid biosynthetic genes to promote saponin biosynthesis in Conyza blinii H. Lév.

  • Wen-Jun Sun
  • Jun-Yi Zhan
  • Tian-Run Zheng
  • Rong Sun
  • Tao Wang
  • Zi-Zhong Tang
  • Tong-Liang Bu
  • Cheng-Lei Li
  • Qi Wu
  • Hui ChenEmail author
Research Article

Abstract

Conyza blinii H. Lév., the most effective component is saponin, is a biennial medicinal material that needs to be overwintered. WRKY transcription factors family is a large protein superfamily that plays a predominant role in plant secondary metabolism, but their characteristics and functions have not been identified in C. blinii. The CbWRKY24 sequence was selected from the transcriptome database of the C. blinii leaves constructed in our laboratory. Phylogenetic tree analysis revealed that it was associated with AaWRKY1 which can regulate artemisinin synthesis in Artemisia annua. Expression analysis in C. blinii revealed that CbWRKY24 was mainly induced by methyl jasmonate (MeJA) and cold treatments. Transcriptional activity assay showed that it had an independent biological activity. Overexpression of CbWRKY24 in transient transformed C. blinii resulted in improved total saponins content, which was attributed to upregulate the expression level of keys genes from mevalonate (MVA) pathway in transient transformed plants compared to wild type (WT) plants. Meanwhile, overexpression the CbWRKY24 in transient transformed tomato fruits showed that the transcript level of related genes in lycopene pathway decreased significantly when compared to WT tomato fruits. Additionally, the MeJA-response-element was found in the promoter regions of CbWRKY24 and the histochemical staining experiments showed that promoter had GUS activity in transiently transformed tobacco leaves. In summary, our results indicated that we may have found a transcription factor that can regulate the biosynthesis of terpenoids in C. blinii.

Keywords

CbWRKY24 gene saponin methyl jasmonate Conyza blinii H. Lév 

Notes

Acknowledgements

We thank all people who participated in this study for their support.

Supplementary material

12041_2018_1026_MOESM1_ESM.doc (27.8 mb)
Supplementary material 1 (doc 28513 KB)

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Copyright information

© Indian Academy of Sciences 2018

Authors and Affiliations

  • Wen-Jun Sun
    • 1
  • Jun-Yi Zhan
    • 1
  • Tian-Run Zheng
    • 1
  • Rong Sun
    • 1
  • Tao Wang
    • 1
  • Zi-Zhong Tang
    • 1
  • Tong-Liang Bu
    • 1
  • Cheng-Lei Li
    • 1
  • Qi Wu
    • 1
  • Hui Chen
    • 1
    Email author
  1. 1.College of life ScienceSichuan Agricultural UniversityYa’anPeople’s Republic of China

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