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Journal of Genetics

, Volume 97, Issue 5, pp 1281–1288 | Cite as

Molecular cloning and spatiotemporal expression of APETALA1-like gene in Lonicera macranthoides

  • Shan Wang
  • Mei Chen Peng
  • Xun Chen
  • Chang Yu Liu
  • Ya Chen
  • Xiang Dan LiuEmail author
  • Ri Bao ZhouEmail author
Research Article
  • 60 Downloads

Abstract

APETALA1 (AP1), a floral meristem identity gene controls the flowering time and floral transition, and plays an important role in inflorescence and floral organ development. The full-length cDNA for AP1 was obtained by rapid amplification of the cDNA ends (RACE) so that the roles of AP1 in Lonicera macranthoides (Lm-AP1) could be better understood. AP1 (accession number in GenBank: MF418642) consisted of a 729-bp open reading frame encoding a protein that contained 242 amino acids, had a deduced molecular mass of 27.9919 kDa and a theoretical isoelectric point of 8.75. No signal peptide or transmembrane domains were detected in the sequences located in the nucleus, but it contained conserved sequences for MADS and the K-box. In the secondary structure, the \(\alpha \)-helix accounts for 60.74%, the \(\beta \)-turn 3.72%. The real-time polymerase chain reaction revealed that AP1 was more highly expressed in flowers, especially at the fourth flowering stage, which implied that it may play a role in flower development. Other L. macranthoides organs, such as stems and leaves, also expressed AP1. This research provided the basis for further analysis of the AP1 functional mechanism during L. macranthoides development.

Keywords

APETALA1 gene bioinformatics fluorescence quantification cloning Lonicera macranthoides Hand.-Mazz 

Notes

Acknowledgements

This work was supported by ‘the Chinese National Natural Science Foundation’ (grant numbers: 81203007 and 81673546), ‘The Natural Science Foundation of China Hunan Province’ (grant number: 2017JJ3237), ‘The Youth Foundation of China Hunan Provincial Education Department’ (grant number: 16B193), ‘The Key Disciplines Foundation Program of Chinese Medicine in China Hunan Province’ (grant number: zy201505), ‘Hunan Province Universities 2011 Collaborative Innovation Center of Protection and Utilization of Hu-xiang Chinese Medicine Resources’ (Xiang teach [2015] no. 351). We thank International Science Editing for editing this manuscript.

Supplementary material

12041_2018_1025_MOESM1_ESM.docx (241 kb)
Supplementary material 1 (docx 240 KB)

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Copyright information

© Indian Academy of Sciences 2018

Authors and Affiliations

  1. 1.College of PharmacyHunan University of Chinese MedicineChangshaPeople’s Republic of China
  2. 2.PharmacyThe Second Affiliated Hospital of Hunan University of Chinese MedicineChangshaPeople’s Republic of China

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