Journal of Genetics

, Volume 97, Issue 5, pp 1155–1168 | Cite as

Roles of Cyclin A, Myc, Jun and Ppm1l in tumourigenic transformation of NIH3T3 cell

  • Cuifang Chang
  • Lingling Xi
  • Jihong ZhangEmail author
  • Weiming Zhao
  • Zhiyou Liu
  • Jianlin Guo
  • Cunshuan Xu
Research Article


To analyse the mechanism of tumourigenic transformation of NIH3T3 cells at the transcriptional level, we used cancerogen 3-methylcholanthrene (3-MCA) and cancerogenic substance phorbol-12-myristate-13-acetate (PMA) to transform NIH3T3 cells and the assessment of transformation was performed using Giemsa staining and methylcellulose colony formation assay. Changes in gene expression profile were detected by Mouse Genome 430 2.0 microarray; and quantitative real-time polymerase chain reaction and Western blotting were used to verify the expression changes of mRNAs and proteins, respectively. With the aid of bioinformatics method, five signalling pathways were identified to participate in different stages of NIH3T3 cell transformation. Further, our study suggested that oncogenes Cyclin A, Myc, Jun and the tumour suppressor gene Ppm1l may play important roles in these pathways.


tumourigenic transformation oncogenes tumour suppressor genes signalling transduction gene expression profile 



This work was supported by the Natural Science Foundation of China (no. 31572270), the Natural Science Foundation of Henan (no. 162300410144) and the Natural Science Foundation of Henan (no. 182300410335).

Supplementary material

12041_2018_1009_MOESM1_ESM.pdf (30 kb)
Supplementary material 1 (pdf 29 KB)


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Copyright information

© Indian Academy of Sciences 2018

Authors and Affiliations

  1. 1.State Key Laboratory Cultivation Base for Cell Differentiation RegulationHenan Normal UniversityXinxiangPeople’s Republic of China
  2. 2.Department of Histology and EmbryologyXinxiang Medical UniversityXinxiangPeople’s Republic of China
  3. 3.Henan Provincial People’s HospitalZhengzhouPeople’s Republic of China

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