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Journal of Genetics

, Volume 97, Issue 5, pp 1131–1138 | Cite as

Deletion / insertion polymorphisms of the prion protein gene (PRNP) in gayal (Bos frontalis)

  • Sameeullah Memon
  • Guozhi Li
  • Heli Xiong
  • Liping Wang
  • Xiangying Liu
  • Mengya Yuan
  • Weidong Deng
  • Dongmei XiEmail author
Research Article
  • 48 Downloads

Abstract

Resistance to fatal disease bovine spongiform encephalopathy (BSE), due to misfolded prion protein in cattle, is associated with a 23-bp indel polymorphism in the putative promoter and a 12-bp indel in intron 1 of the PRNP gene. Gayal (Bos frontalis) is an important semiwild bovid species and of great conservation concern, but till today these indel polymorphisms have not been evaluated in gayals. Therefore, we collected 225 samples of gayals and evaluated the genetic indel polymorphism in the two regions of this PRNP gene. The results revealed high allelic frequencies of insertions at these indel sites: 0.909 and 0.667 for, respectively, the 23 bp and 12 bp indels, both also with significant genotype frequencies (\(\chi ^{2}\): 9.81; 23 bp and \(\chi ^{2}\): 43.56; 12 bp). At the same time, the haplotype data showed indel polymorphisms with extremely low deletion (0.01) in both regions of the PRNP gene. We compared these data with those reported for healthy and BSE-affected cattle (Bos taurus) breeds from two European countries, Germany and Switzerland, and significant difference (\(P\,{<}\,0.001\)) was observed between BSE-affected as well as the healthy cattle. Further, our data were also extensively compared with previous reports on BSE and highly significant (\(P\,{<}\,0.001\)) outcomes were observed. This result suggested negligible genetic susceptibility to BSE in gayals. To the best of our knowledge, this study is the first comprehensive deciphering information about the PRNP indel polymorphisms of 23 bp and 12 bp in gayals, a semiwild species of China.

Keywords

gayal bovine spongiform encephalopathy PRNP gene indels 

Notes

Acknowledgements

The current study was funded by the National Nature Science Foundation of China (grant nos 31460583 and 31101640) and the Yunnan Provincial Key Laboratory of Animal Nutrition and Feed (grant no. DYCX2015004). We thank the two anonymous reviewers for invaluable comments on the manuscript and also appreciate the help of staff (especially Zhijun He and Baqi Su) from the Gongshan County Agriculture, Science and Technology Bureau during sample collection.

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Copyright information

© Indian Academy of Sciences 2018

Authors and Affiliations

  • Sameeullah Memon
    • 1
  • Guozhi Li
    • 1
  • Heli Xiong
    • 1
  • Liping Wang
    • 1
  • Xiangying Liu
    • 1
  • Mengya Yuan
    • 1
  • Weidong Deng
    • 1
  • Dongmei Xi
    • 1
    Email author
  1. 1.Yunnan Provincial Key Laboratory of Animal Nutrition and Feed Science, Faculty of Animal Science and TechnologyYunnan Agricultural UniversityKunmingPeople’s Republic of China

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