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Journal of Genetics

, Volume 97, Issue 5, pp 1451–1456 | Cite as

Genetics of resistance to Cercospora leaf spot disease caused by Cercospora canescens and Psuedocercospora cruenta in yardlong bean (Vigna unguiculata ssp. sesquipedalis) \(\times \) grain cowpea (V. unguiculata ssp. unguiculata) populations

  • Usa Duangsong
  • Kularb Laosatit
  • Prakit SomtaEmail author
  • Peerasak Srinives
Research Note

Abstract

Yardlong bean (Vigna unguiculata ssp. sesquipedalis), a type of cowpea, is an important vegetable legume of Asia. Cercospora leaf spot (CLS) caused by Cercospora canescens and Psuedocercospora cruenta is an important phytopathological problem of the yardlong bean grown in tropical regions. The objectives of this study were to (i) determine mode of inheritance of resistance to CLS caused by C. canescens and P. cruenta, (ii) estimate the heritability of the resistance, (iii) estimate genetic effects on the resistance using six basic populations generated from the cross between the susceptible yardlong bean ‘CSR12906’ and the resistant grain cowpea (V.unguiculata spp. unguiculata) ‘IT90K-59-120’. Segregation for the resistance to both fungi in the F\(_{2}\) population fitted both 3 : 1 ratio and 13 : 3 ratio of susceptible:resistant, while that in the BC2 ((CSR12906\(\times \)IT90K-59-120)\(\times \)IT90K-59-120) population fitted a 1 : 1 ratio, suggesting one recessive gene or two genes with inhibitory gene action control the resistance. Generation mean analysis showed that a simple additive–dominance model was adequate to explain the genetic control of CLS disease resistance, indicating that a single gene controls the resistance. The average number of major genes (effective factors) controlling the resistance was estimated to be 1.05 and 0.92 for C. canescens and P. cruenta, respectively. The broad-sense heritability calculated for resistance to both diseases was higher than 0.90. Altogether, these results indicated that the resistance to CLS disease caused by C. canescens and P. cruenta in grain cowpea IT90K-59-120 is a highly heritable trait governed by a single major recessive gene.

Keywords

cowpea yardlong bean Cercospora leaf spot generation mean analysis Cercospora canescens 

Notes

Acknowledgement

This research was supported by ‘2019 Research Fund’ of the Department of Agronomy, Faculty of Agriculture at Kamphaeng Saen, Kasetsart University.

References

  1. Booker H. M. and Umaharan P. 2008 Quantitative resistance to Cercospora leaf spot disease cause by Pseudocercospora cruenta in cowpea. Euphytica 162, 167–177.CrossRefGoogle Scholar
  2. Castro N. R., Menezes G. C. and Coelho R. S. B. 2003 Inheritance of cowpea resistance to Cercospora leaf spot. Fitopato Bras. 28, 552–554.CrossRefGoogle Scholar
  3. Cavalli L. L. 1952 An analysis of linkage in quantitative inheritance. In Quantitative inheritance (ed. E. C. R. Reevee and C. H. Waddington), pp. 135–144. HMSO, London.Google Scholar
  4. Chankaew S., Somta P., Sorajjapinun W. and Srinives P. 2011 Quantitative trait loci mapping of Cercospora leaf spot resistance in mungbean, Vigna radiata (L.) Wilczek. Mol. Breed. 28, 255–264.Google Scholar
  5. Duangsong U., Kaewwongwal A., Somta P., Chankaew S. and Srinives P. 2016 Identification of a major QTL for resistance to Cercospora leaf spot disease in cowpea (Vigna unguiculata (L.) Walp.) revealed common genomic region with that for the resistance to angular leaf spot in common bean (Phaseolus vulgaris L.). Euphytica  209, 199–207.CrossRefGoogle Scholar
  6. Fery R. L. and Dukes P. D. 1977 An assessment of two genes for Cercospora leaf spot resistance in the Southern pea (Vigna unguiculata (L.) Walp.). J. Hort. Sci. 12, 454–456.Google Scholar
  7. Fery R. L., Dukes P. D. and Cuthbert Jr F. P. 1976 The inheritance of Cercospora leaf spot resistance in Southern pea (Vigna unguiculata (L.) Walp.). J. Hort. Sci. 101, 148–149.Google Scholar
  8. Fery R. L., Dukes P. D. and Cuthbert Jr F. P. 1977 Yield-loss of Southern Pea (Vigna unguiculata) caused by Cercospora leaf spot. Plant Dis. 61, 741–743.Google Scholar
  9. Lande R. 1981 The minimum number of genes contributing to quantitative variation between and within populations. Genetics  99, 541–553.PubMedPubMedCentralGoogle Scholar
  10. Mather K. and Jinks J. L. 1982 Biometrical genetics. Chapman & Hall, London.CrossRefGoogle Scholar
  11. R Development Core Team 2013 R: A language and environment for statistical computing. R Foundation for Statistical Computing, Vienna.Google Scholar
  12. Schneider R. W., Williams R. J. and Sinclair J. B. 1976 Cercospora leaf spot of cowpea: models for estimating yield loss. Phytopathology 66, 384–388.CrossRefGoogle Scholar
  13. Simko I. and Piepho H. P. 2012 The area under the disease progress stairs: calculation, advantage, and application. Phytopathology 102, 381–389.CrossRefGoogle Scholar
  14. Warner J. N. 1952 A method for estimating heritability. Agron. J. 44, 427–430.CrossRefGoogle Scholar
  15. Wright S. 1968 Evolution and the genetics of populations: genetic and biometric foundations, vol. 1. University of Chicago Press, Chicago.Google Scholar

Copyright information

© Indian Academy of Sciences 2018

Authors and Affiliations

  1. 1.Faculty of Agriculture, Department of AgronomyKasetsart UniversityNakhon Pathom Thailand
  2. 2.Center for Advanced Studies for Agriculture and Food (CASAF)Kasetsart University Institute for Advanced Studies, Kasetsart UniversityBangkok Thailand
  3. 3.Royal Society of ThailandBangkok Thailand

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