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Isolation and characterization of microsatellite markers in the spiny lobster, Panulirus echinatus Smith, 1869 (Decapoda: Palinuridae) by Illumina MiSeq sequencing

  • Michelli F. Santos
  • Isis G. B. Souza
  • Sulimary O. Gomes
  • Geice R. Silva
  • Paul Bentzen
  • Fabio M. Diniz
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Introduction

The isolation and characterization of 17 polymorphic microsatellite loci from the spiny lobster Panulirus echinatus (Decapoda: Palinuridae) by Illumina MiSeq sequencing is described. The analysis of genetic variability was performed in 42 individuals from 3 different populations (Saint Peter and Saint Paul Rocks, The Rocas Atoll and Cape Verde). The number of alleles ranged from 2 to 8, with an average of 5.4 ± 1.4 alleles per locus. The values of allelic richness varied from 1.8 to 6.2 (average of 3.9 ± 1.0), while PIC values ranged between 0.173 and 0.811. Overall mean observed and expected heterozygosity was estimated to be 0.418 ± 0.165 and 0.587 ± 0.173, respectively. These are the first microsatellite markers characterized for P. echinatus and it is expected that these markers will help to obtain the necessary information for developing conservation and management strategies for this highly valuable fishery species.

Lobster fisheries, undoubtedly, is one of the most...

Keywords

molecular markers MiSeq sequencing brown spiny lobster population genetics 

Notes

Acknowledgements

Sequencing was done on a MiSeq DNA sequencer purchased with a bequest from Elizabeth Ann Nielsen to the Marine Gene Probe Laboratory. The authors acknowledge financial support from the Brazilian Federal Government (Science without Borders programme) scholarship. The authors MFS, IGBS, SOG and GRS were supported by scholarships from the National Council for Scientific and Technological Development (CNPq).

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Copyright information

© Indian Academy of Sciences 2018

Authors and Affiliations

  • Michelli F. Santos
    • 1
    • 2
  • Isis G. B. Souza
    • 1
    • 2
  • Sulimary O. Gomes
    • 1
    • 2
  • Geice R. Silva
    • 1
    • 2
  • Paul Bentzen
    • 3
  • Fabio M. Diniz
    • 4
    • 5
  1. 1.Universidade Federal do PiauíTeresinaBrazil
  2. 2.Universidade Federal do Piauí, Northeast Biotechnology Network RENORBIOTeresinaBrazil
  3. 3.Department of BiologyDalhousie UniversityHalifaxCanada
  4. 4.Empresa Brasileira de Pesquisa Agropecuária, Embrapa Meio-NorteTeresinaBrazil
  5. 5.Empresa Brasileira de Pesquisa Agropecuária, Embrapa Caprinos e OvinosSobralBrazil

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