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Aleurothrixus trachoides (Back) can transmit begomovirus from Duranta to potato, tomato and bell pepper

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Abstract

Solanum whitefly, Aleurothrixus trachoides (Back). (Hemiptera: Aleyrodidae) was considered as a non-virus vector by European and Mediterranean Plant Protection Organization (EPPO) reports. However, in the present study it was found to transmit Duranta leaf curl virus (DLCV) to tomato, bell pepper and potato. A. trachoides infested field samples of Duranta sp (100%) and tomato (20%) tested positive for begomovirus by PCR using begomovirus degenerate primers and primers specific to Tomato leaf curl New Delhi virus showing amplicon of 520 bp and 2.7 Kb respectively. The DNA samples of A. trachoides collected from virus positive duranta and tomato plants also tested positive for the virus. Virulent whiteflies from duranta could successfully transmit DLCV to bell pepper (26%) and tomato (13%) plants as confirmed by Rolling Circle Amplification. The rate of virus transmission by A. trachoides from DLCV inoculated tomato to bell pepper and tomato to potato was 100% and tomato to tomato was 80%. The results suggest whitefly A. trachoides as the vector for DLCV and to the best of our knowledge, this is the first report for A. trachoides as vector of begomovirus. These findings suggest need for reconsideration of A. trachoides as a virus-vector. This will have great impact on solanaceous vegetable cultivation in India and other parts of the world.

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Acknowledgements

This study supported by SERB Project No: EEQ/2016/000272. Authors are thankful to Dr. A.K. Dubey, Zoological Survey of India, Andaman & Nicobar Regional Centre Haddo, Port Blair 744 102, for identification of whitefly samples.

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Authors and Affiliations

  1. ICAR-Indian Agricultural Research Institute, Regional Station, Pune, India

    K Chandrashekar, Ashutosh Rao, Raj Verma & Savarni Tripathi

  2. Department of Botany, Savitribai Phule Pune University, Pune, India

    Asawari Gorane

Authors
  1. K Chandrashekar
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  2. Ashutosh Rao
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  3. Asawari Gorane
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  4. Raj Verma
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  5. Savarni Tripathi
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Corresponding author

Correspondence to K Chandrashekar.

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Communicated by Manchikatla Venkat Rajam

Corresponding editor: Manchikatla Venkat Rajam

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Chandrashekar, K., Rao, A., Gorane, A. et al. Aleurothrixus trachoides (Back) can transmit begomovirus from Duranta to potato, tomato and bell pepper. J Biosci 45, 36 (2020). https://doi.org/10.1007/s12038-020-0012-2

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