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Target-specific gene delivery in plant systems and their expression: Insights into recent developments

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Abstract

In order to improve crop plants in terms of their yield, drought resistance, pest resistance, nutritional value, etc., modern agriculture has relied upon plant genetic engineering. Since the advent of recombinant DNA technology, several tools have been used for genetic transformations in plants such as Agrobacterium tumefaciens, virus-mediated gene transfer, direct gene transfer systems such as electroporation, particle gun, microinjection and chemical methods. All these traditional methods lack specificity and the transgenes are integrated at random sites in the plant DNA. Recently novel techniques for gene targeting have evolved such as engineered nucleases such as Zinc Finger Nucleases, Transcription Activator like effector nucleases, Clustered regular interspaced short palindromic repeats. Other advances include improvement in tools for delivery of gene editing components which include carrier proteins, and carbon nanotubes. The present review focuses on the latest techniques for target specific gene delivery in plants, their expression and future directions in plant biotechnology.

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  1. CSIR-Indian Institute of Chemical Biology, Jadavpur, Kolkata, 700 032, India

    Debdyuti Nandy

  2. Department of Microbiology, St. Xavier’s College, Kolkata, 700 016, India

    Amrita Maity & Arup Kumar Mitra

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  1. Debdyuti Nandy
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Communicated by MANCHIKATLA VENKAT RAJAM

Corresponding editor: Manchikatla Venkat Rajam

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Nandy, D., Maity, A. & Mitra, A.K. Target-specific gene delivery in plant systems and their expression: Insights into recent developments. J Biosci 45, 30 (2020). https://doi.org/10.1007/s12038-020-0008-y

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