Human placental laminin: Role in neuronal differentiation, cell adhesion and proliferation


Human placental extract has wound healing potential. Immuno-blots revealed presence of laminin in placental extract (70 ± 0.257 µg/ml; n=3). It was purified using immuno-affinity chromatography. SDS-PAGE and SE-HPLC indicated a188 kDa protein with some small peptides. Since placental laminin existed in its truncated form, its roles in cellular migration, differentiation and wound healing were verified. Induction of cellular migration and motility in rat fibroblasts were enhanced by placental laminin as observed from scratch wound assay. Promotion of neuronal differentiation of PC12 cells by placental laminin was observed by phase contrast microscopy. Confocal images showed presence of laminin on the cell surface and along the axonal processes. Significant interaction between integrin receptors and laminin responsible for cellular differentiation was demonstrated from co-localization experiments. Union between integrin receptor and its synthetic antagonist revealed retarded pattern of neurite outgrowth in laminin treated cells. Animal model studies revealed faster wound healing in the presence of placental laminin. Induction of re-epithelialization and angiogenesis in wound area by cellular proliferation and adhesion were observed. The cytokine levels showed an initial rise and gradual fall over the duration of wound healing on application of the fragmented laminin. Thus, roles of placental laminin in neuronal differentiation and wound healing were indicated.

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alkaline phosphatase


5-bromo-4-chloro-3-indolyl-phosphate/nitro blue tetrazolium


bovine serum albumin


corticotrophin releasing factor


1-ethyl-3-(3-dimethylaminopropyl) carbodiimide, HCl


the biomolecule as described in textbooks




2-(N-morpholino) ethansulfonic acid


nicotinamide adenine dinucleotide phosphate


nitro blue tetrazolium


nerve growth factor


phosphate buffered saline, pH 7.4

Placental laminin:

fragmented laminin from an aqueous extract from human placenta




Serum glutamic pyruvic transaminase


serum glutamic oxaloacetic transaminase


soyabean trypsin inhibitor




tumor growth factor


tumor necrosis factor-α

Standard laminin:

full-length human placental laminin from Sigma


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CM and SS were Senior Research Fellows, CSIR, New Delhi, India. SD was supported by CSIR–Network Project miND-BSC 0115. The technical help rendered by Diptodeep Sirkar, Sounak Bhattacharya and Dipayan De is acknowledged for their contributions in confocal and super-resolution microscopy and quantification of confocal image measurements respectively.

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Correspondence to Debasish Bhattacharyya.

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Corresponding editor: Aurnab Ghose

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Mukherjee, C., Saleem, S., Das, S. et al. Human placental laminin: Role in neuronal differentiation, cell adhesion and proliferation. J Biosci 45, 93 (2020).

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  • Cell adhesion
  • human placenta
  • laminin
  • neurite outgrowth
  • wound healing