Journal of Biosciences

, Volume 43, Issue 2, pp 351–364 | Cite as

Deletion of Dictyostelium discoideum Sir2A impairs cell proliferation and inhibits autophagy

  • Rakhee Lohia
  • Punita Jain
  • Mukul Jain
  • Himanshu Mishra
  • Pradeep Kumar Burma
  • Anju Shrivastava
  • Shweta Saran


Sirtuins are a family of deacetylases (Class III histone deacetylases) with evolutionarily conserved functions in cellular metabolism and chromatin regulation. Out of the seven human Sirtuins, the function of Sirt2 is the least understood. The purpose of the present study was to investigate the role of Sir2A, a homolog of human Sirt2 in Dictyostelium discoideum (Dd), a lower eukaryote. We created both overexpressing and deletion strains of Ddsir2A to analyse its functions. We observed sir2A mRNA expression throughout development and the transcript was present in the prespore/spore region of multicellular structures developed. They show a preference towards prestalk/stalk pathway when co-developed with wild-type cells during chimera formation. Deletion strain showed a multi-tipped phenotype, decrease in cell proliferation and inhibition of autophagy. In conclusion, our results show low cAMP levels, reduced cell-adhesion, weak cell migration and impaired autophagy to be responsible for the phenotype shown by the null cells. This study provides new insights into the functions of Ddsir2A.


Autophagy development Dictyostelium patterning sir2A 



SS thanks DST (SR/SO/BB-18/2008) and ICMR (54/41/CFP/GER/2011-NCD-II) for research grants. Partial research grants from DST-PURSE, UGC networking, UPE-II and DST-FISTII are also acknowledged. RL, PJ, HM and MJ thank CSIR, DST-INSPIRE, CSIR and DBT for fellowships, respectively.

Supplementary material

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Supplementary material 1 (PDF 2247 kb)


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Copyright information

© Indian Academy of Sciences 2018

Authors and Affiliations

  1. 1.School of Life SciencesJawaharlal Nehru UniversityNew DelhiIndia
  2. 2.Department of GeneticsUniversity of DelhiNew DelhiIndia
  3. 3.Department of ZoologyUniversity of DelhiNew DelhiIndia

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