In vitro bioactivity evaluation of \(\upalpha \)-calcium sulphate hemihydrate and bioactive glass composites for their potential use in bone regeneration

  • Yanyan Zheng
  • Chengdong Xiong
  • Dujuan Zhang
  • Lifang Zhang


To combine the self-setting property of \(\upalpha \)-calcium sulphate hemihydrate (\(\upalpha \)-CSH) with the bioactive property of bioactive glass (BG), BG was added into \(\upalpha \)-CSH to prepare \(\upalpha \)-CSH/BG composites. The in vitro bioactivity and cytocompatibility of the \(\upalpha \)-CSH/BG composites were assessed by soaking the composites in simulated body fluid (SBF) and co-culturing with the osteoblasts, respectively. Formation of a bone-like apatite layer on the composite surface was studied by X-ray diffraction (XRD), Fourier transform infrared spectrometry (FTIR), scanning electron microscopy (SEM) and energy-dispersive spectroscopy (EDS). Variations in ionic concentration and pH of the SBF solution were detected. The incorporation of BG into \(\upalpha \)-CSH, effectively compensated for the pH decrease caused by the dissolution of \(\upalpha \)-CSH and the ion exchange. Osteoblast-like cells (MG63) were cultured on the samples, and the MTT results confirmed that the composites containing BG were more favourable for the proliferation of these cells. Hence, \(\upalpha \)-CSH/BG composites might have great potential for the use as a bone regeneration material.


Bioactive glass \(\upalpha \)-calcium sulphate hemihydrate composite bioactivity cytocompatibility 



This work was supported financially by the Doctoral Program of North Sichuan Medical College (No. CBY16-QD-02), the North Sichuan Medical College Scientific Research and Development Projects (No. CBY16-A-ZD02), and the key project of ‘Western Light’ Foundation of Chinese Academy of Sciences.


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Copyright information

© Indian Academy of Sciences 2018

Authors and Affiliations

  1. 1.School of Basic Medical SciencesNorth Sichuan Medical CollegeNanchongPeople’s Republic of China
  2. 2.Chengdu Institute of Organic ChemistryChinese Academy of SciencesChengduPeople’s Republic of China

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