Application of Various Delivery Methods for CRISPR/dCas9

Abstract

As gene-editing technology has become more and more popular in the life sciences, CRISPR has brought good news to scientific researchers because of its efficiency, convenience, and wide application. Its wide application has also promoted the development of basic scientific research, agriculture, basic medicine, and clinical treatment. However, how the CRISPR/dCas9 system is effectively delivered to the target organs or cells is still unknown. This paper briefly introduces the CRISPR/dCas9 system and then lists some common delivery methods and their characteristics.

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Acknowledgment

We thank the National Key Specialty Construction Project of Clinical Pharmacy (Grant No. 30305030698), the Science & Technology Program of Sichuan Province (Grant Nos. 2009SZ0226, 2014FZ0103, 2015JQO027, 2015ZR0160, 20ZDYF1490, and 20CXTD0043), the Health Department of Sichuan Province Grant Nos. 100491, 120111, and 17ZD038), Sichuan Cancer Hospital (Grant No. YB2019001), Chengdu City Science and Technology Project (Grant No. 11PPYB010SF-289), the Young Scholars Foundation of Sichuan Provincial People’s Hospital (Grant Nos. 30305030606 and 30305030859), the Cadre Health Care Research Project of Sichuan Province (Grant No. 2019-801), and Zambon Pharmaceutical Scientific Research Foundation of the Chengdu Pharmaceutical Association (Grant No. 201905).

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Liu, Z., Liao, Z., Chen, Y. et al. Application of Various Delivery Methods for CRISPR/dCas9. Mol Biotechnol (2020). https://doi.org/10.1007/s12033-020-00258-8

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Keywords

  • CRISPR
  • dCas9
  • Delivery