The purpose of this study was to construct a biomimetic urethral repair substitute. The nano-Laponite/polylactic acid–glycolic acid copolymer (PLGA) fiber scaffolds were produced to replicate the natural human urethra tissue microenvironment. PLGA (molar ratio 50:50) and Laponite were used in this study as raw materials. The nano-Laponite/PLGA scaffolds were fabricated via electrospinning technology. After preparing the material, the microstructural and mechanical properties of the nano-Laponite/PLGA scaffold were tested via scanning electron microscopy and electronic universal testing. The effects of different amounts of Laponite on the degradation of the nano-Laponite/PLGA scaffold were studied. Human umbilical vein endothelial cells (HUVECs) were co-cultured with PLGA and nano-Laponite/PLGA scaffolds for 24, 48, or 72 h. Scanning electron microscopy results illustrated that the microstructure of the scaffold fabricated by electrospinning was similar to that of the natural extracellular matrix. When the electrospinning liquid contained 10% Laponite, the nano-Laponite/PLGA stress–strain curve illustrated that the scaffold has strong elastic deformation ability. HUVECs exhibited good growth on the nano-Laponite/PLGA scaffold. When the scaffold contained 1% Laponite, the cell proliferation rate in the CCK-8 test was significantly better than that for the other three materials, displaying good cell culture characteristics. The 1% nano-Laponite/PLGA composite scaffold can be used as a suitable urethral repair material, but its performance requires further development and research.
This is a preview of subscription content, access via your institution.
Buy single article
Instant access to the full article PDF.
Tax calculation will be finalised during checkout.
Subscribe to journal
Immediate online access to all issues from 2019. Subscription will auto renew annually.
Tax calculation will be finalised during checkout.
Yang, B., Peng, B., & Zheng, J. (2011). Cell-based tissue-engineered urethras. Lancet, 378(9791), 568–569.
Guan, Y., Ou, L., Hu, G., Wang, H., Xu, Y., Chen, J., et al. (2008). Tissue engineering of urethra using human vascular endothelial growth factor gene-modified bladder urothelial cells. Artificial Organs, 32(2), 91–99.
Williamson, M. R., Black, R., & Kielty, C. (2006). PCL-PU composite vascular scaffold production for vascular tissue engineering: attachment, proliferation and bioactivity of human vascular endothelial cells. Biomaterials, 27(19), 3608–3616.
Ghasemi-Mobarakeh, L., Prabhakaran, M. P., Morshed, M., Nasr-Esfahani, M. H., & Ramakrishna, S. (2008). Electrospun poly(epsilon-caprolactone)/gelatin nanofibrous scaffolds for nerve tissue engineering. Biomaterials, 29(34), 4532–4539.
Eberli, D., Freitas Filho, L., Atala, A., & Yoo, J. J. (2009). Composite scaffolds for the engineering of hollow organs and tissues. Methods (San Diego, CA), 47(2), 109–115.
Selim, M., Bullock, A. J., Blackwood, K. A., Chapple, C. R., & MacNeil, S. (2011). Developing biodegradable scaffolds for tissue engineering of the urethra. BJU International, 107(2), 296–302.
Fossum, M., & Nordenskjold, A. (2010). Tissue-engineered transplants for the treatment of severe hypospadias. Hormone Research in Paediatrics, 73, 148–152.
Kalantari, K., Afifi, A. M., Jahangirian, H., & Webster, T. J. (2019). Biomedical applications of chitosan electrospun nanofibers as a green polymer: Review. Carbohydrate Polymers, 207, 588–600.
Jia, W., Tang, H., Wu, J., Hou, X., Chen, B., Chen, W., et al. (2015). Urethral tissue regeneration using collagen scaffold modified with collagen binding VEGF in a beagle model. Biomaterials, 69, 45–55.
Haider, A., Gupta, K. C., & Kang, I.-K. (2014). title. Nanoscale Research letters, 9(1), 314.
Wang, J.-H., Xu, Y.-M., Fu, Q., Song, L.-J., Li, C., Zhang, Q., et al. (2013). Continued sustained release of VEGF by PLGA nanospheres modified BAMG stent for the anterior urethral reconstruction of rabbit. Asian Pacific Journal of Tropical Medicine, 6(6), 481–484.
Gaharwar, A. K., Schexnailder, P. J., Dundigalla, A., White, J. D., Matos-Pérez, C. R., Cloud, J. L., et al. (2011). Highly extensible bio-nanocomposite fibers. Macromolecular Rapid Communications, 32(1), 50–57.
Schexnailder, P. J., Gaharwar, A. K., Bartlett, R. L., II, Seal, B. L., & Schmidt, G. (2010). Tuning cell adhesion by incorporation of charged silicate nanoparticles as cross-linkers to polyethylene oxide. Macromolecular Bioscience, 10(12), 1416–1423.
Dawson, J. I., Kanczler, J. M., Yang, X. B., Attard, G. S., & Oreffo, R. O. (2011). Clay gels for the delivery of regenerative microenvironments. Advanced Materials (Deerfield Beach, Fla.), 23(29), 3304–3308.
Chen, D., Sun, K., Mu, H., Tang, M., Liang, R., Wang, A., et al. (2012). title. International Journal of Nanomedicine, 7, 2621.
Lee, S. J., Heo, D. N., Moon, J. H., Ko, W. K., Lee, J. B., Bae, M. S., et al. (2014). Electrospun chitosan nanofibers with controlled levels of silver nanoparticles. Preparation, characterization and antibacterial activity. Carbohydrate Polymers, 111, 530–537.
Wang, F., Sun, Z., Yin, J., & Xu, L. (2019). Preparation, characterization and properties of porous PLA/PEG/curcumin composite nanofibers for antibacterial application. Nanomaterials (Basel, Switzerland), 9(4).
Lv, X., Guo, Q., Han, F., Chen, C., Ling, C., Chen, W., & Li, B. (2016). Electrospun poly(l-lactide)/poly(ethylene glycol) scaffolds seeded with human amniotic mesenchymal stem cells for urethral epithelium repair. International Journal of Molecular Sciences, 17(8).
Wang, K., Guo, C., Dong, X., Yu, Y., Wang, B., Liu, W., et al. (2018). In vivo evaluation of reduction-responsive alendronate-hyaluronan-curcumin polymer-drug conjugates for targeted therapy of bone metastatic breast cancer. Molecular Pharmaceutics, 15(7), 2764–2769.
Chen, D., Lian, S., Sun, J., Liu, Z., Zhao, F., Jiang, Y., et al. (2016). Design of novel multifunctional targeting nano-carrier drug delivery system based on CD44 receptor and tumor microenvironment pH condition. Drug Delivery, 23(3), 798–803.
Gaharwar, A. K., Mukundan, S., Karaca, E., Dolatshahi-Pirouz, A., Patel, A., Rangarajan, K., et al. (2014). Nanoclay-enriched poly(varepsilon-caprolactone) electrospun scaffolds for osteogenic differentiation of human mesenchymal stem cells. Tissue Engineering Part A, 20(15-16), 2088–2101.
Zhou, S., Yang, R., Zou, Q., Zhang, K., Yin, T., Zhao, W., et al. (2017). Fabrication of tissue-engineered bionic urethra using cell sheet technology and labeling by ultrasmall superparamagnetic iron oxide for full-thickness urethral reconstruction. Theranostics, 7(9), 2509–2523.
Liang, M., Li, Z., Gao, C., Wang, F., & Chen, Z. (2018). Preparation and characterization of gelatin/sericin/carboxymethyl chitosan medical tissue glue. Journal of Applied Biomaterials & Functional Materials, 16(2), 97–106.
This study was financially supported by grants from the Guangzhou Women and Children Medical Center (No. 0170006), Taishan Young Scholar Program (No. qnts20161035), and Shandong Provincial Natural Science Foundation for Outstanding Young Scholar (No. ZR2019YQ30), Shandong Provincial Natural Science Foundation for Key Basic Research (No. ZR2019ZD24).
Conflict of interest
The authors have no financial conflicts of interest.
No animal experiments were performed for this article.
Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations.
About this article
Cite this article
Wang, Z., Hu, J., Yu, J. et al. Preparation and Characterization of Nano-Laponite/PLGA Composite Scaffolds for Urethra Tissue Engineering. Mol Biotechnol 62, 192–199 (2020). https://doi.org/10.1007/s12033-020-00237-z
- Urethral stent