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Molecular Cloning and Biochemical Characterization of Iron Superoxide Dismutase from Leishmania braziliensis

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Abstract

Leishmaniasis is one of the most important neglected tropical diseases, with a broad spectrum of clinical manifestations. Among the clinical manifestations of the disease, cutaneous leishmaniasis, caused by species of Leishmania braziliensis, presents wide distribution in Brazil. In this work, we performed the cloning, expression, and purification of the enzyme superoxide dismutase of Leishmania braziliensis (LbSOD-B2) considered a promising target for the search of new compounds against leishmaniasis. In vitro assays based on pyrogallol oxidation showed that LbSOD-B2 is most active around pH 8 and hydrogen peroxide is a LbSOD-B2 inhibitor at low millimolar range (IC50 = 1 mM).

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Acknowledgements

The authors are grateful for the academic support of PPGBiotec-UEFS and the Grants from CNPq 306277/2014-0, FAPESB BOL0688/2014, and FAPESP 2013/07600-3.

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Authors and Affiliations

  1. Programa de pós-graduação em Biotecnologia, Universidade Estadual de Feira de Santana, Feira de Santana, BA, Brazil

    Camila C. B. Brito & Marcelo S. Castilho

  2. Sao Carlos Institute of Physics, University of Sao Paulo, Av. Joao Dagnone, 1100 Jardim Santa Angelina, São Carlos, SP, 13563-120, Brazil

    Fernando V. Maluf, Gustavo M. A. de Lima & Rafael V. C. Guido

  3. Faculdade de Farmácia, Universidade Federal da Bahia, Salvador, BA, 40170-290, Brazil

    Marcelo S. Castilho

  4. MAX IV Laboratory, Lund University, PO Box 118, 221 00, Lund, Sweden

    Gustavo M. A. de Lima

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  1. Camila C. B. Brito
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Correspondence to Marcelo S. Castilho.

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Brito, C.C.B., Maluf, F.V., de Lima, G.M.A. et al. Molecular Cloning and Biochemical Characterization of Iron Superoxide Dismutase from Leishmania braziliensis. Mol Biotechnol 60, 595–600 (2018). https://doi.org/10.1007/s12033-018-0095-2

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