Abstract
Carbonic anhydrases (CAs) are a class of zinc-containing metalloenzymes that can reversibly catalyse the hydration reaction of carbon dioxide. Antarctic algae are the most critical component of the Antarctic ecosystem; algae can enter the carbon cycle food chain by fixing carbon dioxide from the air. In this study, the complete open reading frames (ORFs) of CA1 (GenBank ID KY826431), CA2 (GenBank ID KY826432), and CA3 (GenBank ID KY826433), encoding CAs in the Antarctic ice microalga Chlamydomonas. sp. ICE-L, were successfully cloned using reverse transcription-polymerase chain reaction (RT-PCR). In addition, the expression patterns of CAs under blue light, under UV light, and in the dark were determined by quantitative reverse transcription-polymerase chain reaction (qRT-PCR). The CA1, CA2, and CA3 ORFs encode proteins of 376, 430, and 419 amino acids, respectively. Phylogenetic analysis revealed that all amino acid sequences showed high homology with those of C. sp. ICE-L. There are six types of algal CAs; we hypothesised that the CAs studied here are most likely α-CAs. Expression analysis showed that the transcription level of the CAs was influenced by both UV light and blue light. These findings provide additional insight into the molecular mechanisms of CAs and will accelerate the development of CAs for applications in agriculture and environmental governance.
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Acknowledgements
This work was supported by the National Key Research and Development Program of China (Grant No. 2018YFD0900705; 2018YFD0901103), the Natural Science Foundation of China (Grant Nos. 41576187, No. 41776203, No. 21576145), Key Research and Development Program of Shandong Province (Grant Nos. 2016YYSP017, No. 2016ZDJS06A03, No. 2017GHY15112, No. 2018YYSP024, Grant No. 2018GHY115034), Public Science and Technology Research Funds Projects of Ocean (Grant No. 201405015), Deep Sea Biological Resources Plan of China (Grant No. DY135-B2-14), Qingdao Entrepreneurship & Innovation Pioneers Program (Grant No. 15-10-3-15-(44)-zch), and Ningbo Public Service Platform for High-Value Utilization of Marine Biological Resources (Grant No. NBHY-2017-P2).
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Shi, C., An, M., Miao, ·. et al. Isolation and Expression Analysis of Three Types of α-Carbonic Anhydrases from the Antarctic Alga Chlamydomonas sp. ICE-L under Different Light Stress Treatments. Mol Biotechnol 61, 200–208 (2019). https://doi.org/10.1007/s12033-018-00152-4
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DOI: https://doi.org/10.1007/s12033-018-00152-4