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Glial-Like Differentiation Potential of Human Mature Adipocytes

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Abstract

The potential ability to differentiate dedifferentiated adipocytes into a neural lineage is attracting strong interest as an emerging method of producing model cells for the treatment of a variety of neurological diseases. Here, we describe the efficient conversion of dedifferentiated adipocytes into a neural-like cell population. These cells grew in neurosphere-like structures and expressed a high level of the early neuroectodermal marker Nestin. These neurospheres could proliferate and express stemness genes, suggesting that these cells could be committed to the neural lineage. After neural induction, NeuroD1, Sox1, Double Cortin, and Eno2 were not expressed. Patch clamp data did not reveal different electrophysiological properties, indicating the inability of these cells to differentiate into mature neurons. In contrast, the differentiated cells expressed a high level of CLDN11, as demonstrated using molecular method, and stained positively for the glial cell markers CLDN11 and GFAP, as demonstrated using immunocytochemistry. These data were confirmed by quantitative results for glial cell line-derived neurotrophic factor production, which showed a higher secretion level in neurospheres and the differentiated cells compared with the untreated cells. In conclusion, our data demonstrate morphological, molecular, and immunocytochemical evidence of initial neural differentiation of mature adipocytes, committing to a glial lineage.

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Authors and Affiliations

  1. Clinica di Ematologia, Dipartimento di Scienze Cliniche e Molecolari, Università Politecnica delle Marche, Via Tronto, 60020, Ancona, Italy

    Antonella Poloni, Giulia Maurizi, Federica Foia, Domenico Mattiucci, Stefania Mancini, Attilio Olivieri & Pietro Leoni

  2. Dipartimento di Medicina Sperimentale e Clinica, Università Politecnica delle Marche, Ancona, Italy

    Eleonora Mondini & Saverio Cinti

  3. Dipartimento di Scienze della Terra, della Vita e dell’Ambiente, Sezione di Fisiologia, Università di Urbino Carlo Bo, Urbino, Italy

    Patrizia Ambrogini & Davide Lattanzi

  4. Clinica Chirurgia del Pancreas, Università Politecnica delle Marche, Ospedali Riuniti, Ancona, Italy

    Massimo Falconi

Authors
  1. Antonella Poloni
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  2. Giulia Maurizi
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  3. Federica Foia
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  4. Eleonora Mondini
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  5. Domenico Mattiucci
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  6. Patrizia Ambrogini
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  7. Davide Lattanzi
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  8. Stefania Mancini
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  9. Massimo Falconi
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  10. Saverio Cinti
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  11. Attilio Olivieri
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  12. Pietro Leoni
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Corresponding author

Correspondence to Antonella Poloni.

Additional information

The authors Antonella Poloni and Giulia Maurizi contributed equally to the work.

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Poloni, A., Maurizi, G., Foia, F. et al. Glial-Like Differentiation Potential of Human Mature Adipocytes. J Mol Neurosci 55, 91–98 (2015). https://doi.org/10.1007/s12031-014-0345-0

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  • DOI: https://doi.org/10.1007/s12031-014-0345-0

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