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Evidences for B6C3-Tg (APPswe/PSEN1dE9) Double-Transgenic Mice Between 3 and 10 Months as an Age-Related Alzheimer’s Disease Model

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Abstract

Transgenic mouse has shown great advantages in the study of Alzheimer’s disease (AD) and drug screening as AD develops rapidly resent years, while more detail information of these transgenic mice and experience of application are needed. To obtain the basic background information of the B6C3-Tg (APPswe/PSEN1dE9) double-transgenic mouse, which was reported with early onset AD, three- to ten-month-old B6C3-Tg AD mice and normal C57BL/6 mice were selected randomly to test the ability of learning memory by Morris water maze, the brain acetylcholinesterase (AChE) activity by AChE kit, and beta amyloid protein level by immunohistochemistry staining. Compared with the control group, the escape latency time of B6C3-Tg AD mice at 9 and 10 months of age is significantly longer (P < 0.05) in Morris maze test, and the activity of brain AChE is higher. β-Amyloid plaques were observed at 3 months of age and developed rapidly. Statistical analysis showed a positive correlation between the area of these plaques and the ages of B6C3-Tg AD mouse (y = 0.0355e0.5557x, R = 0.9557). The model’s behavior is conformed to simulate behaviors of human Alzheimer’s disease at the early stage and may provide detail background information a new choice when transgenic mice are needed in the research of AD.

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Acknowledgments

This work was supported by Science and Technology Planning Project of Guangdong Province, China (2011B010500013); Foundation of Foshan Cooperation Project, Guangdong Province, China; (2011BY100102) and the Foundation for Medical Science Research of Guangdong Province, China (A2012130). We thank Zhichang Wan (The Chinese University of Hong Kong) and Xianglu Rong (Guangzhou University of Chinese Medicine) for the editorial assistance in the preparation of this manuscript.

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Authors and Affiliations

  1. Southern Medical University, Guangzhou, Guangdong Province, China

    Zhiyong Zhong & Xiaojiang Tang

  2. Guangdong Medical Laboratory Animal Center, Foshan, Guangdong Province, China

    Zhiyong Zhong, Lin Yang, Xiansheng Wu, Wei Huang, Jiarong Yan, Shenglai Liu, Xia Sun, Ke Liu, Shaosong Kuang & Xiaojiang Tang

  3. The Chinese University of Hong Kong, Sha Tin, Hong Kong

    Huangquan Lin

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  1. Zhiyong Zhong
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  2. Lin Yang
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  4. Wei Huang
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Correspondence to Xiaojiang Tang.

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Zhong, Z., Yang, L., Wu, X. et al. Evidences for B6C3-Tg (APPswe/PSEN1dE9) Double-Transgenic Mice Between 3 and 10 Months as an Age-Related Alzheimer’s Disease Model. J Mol Neurosci 53, 370–376 (2014). https://doi.org/10.1007/s12031-013-0203-5

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  • DOI: https://doi.org/10.1007/s12031-013-0203-5

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