The role of Lnc_OC1 in gastric cancer (GC) has not been well documented. The very purpose of the present study was to determine not only the expression of Lnc_OC1 in gastric tissues but also its role in the formation of GC. Furthermore, the expression levels of Lnc-OC1 were examined in H. pylori-positive versus H. pylori-negative GC tissues.
Tumor and adjacent normal tissues were collected from 43 patients with GC. RNA extraction and cDNA synthesis were performed. Then, qRT-PCR was carried out. Finally, an independent sample t test was run to examine the expression level of Lnc_OC1 in a GC and normal tissues using SPSS program.
The results revealed a significantly higher expression level of Lnc_OC1 in the GC tissues as compared with the normal tissues (p = 0.0037). The correlations between the expression level of Lnc_OC1 and the clinical features of patients were not statistically significant (p > 0.05). Moreover, the expression of Lnc-OC1 was significantly higher in H. pylori-positive patients as compared with H. pylori-negative patients (p = 0.01).
The findings of the present study revealed that deregulation of Lnc_OC1 may have a role in the H. pylori-associated pathogenesis of GC. Moreover, a direct association can be speculated between GC formation and Lnc_OC1 expression. The mentioned findings highlight the potential role of Lnc_OC1 as a prognostic biomarker of GC. Hence, further evaluations are required in this respect.
This is a preview of subscription content, log in to check access.
Buy single article
Instant access to the full article PDF.
Price includes VAT for USA
Subscribe to journal
Immediate online access to all issues from 2019. Subscription will auto renew annually.
This is the net price. Taxes to be calculated in checkout.
Sitarz R, Skierucha M, Mielko J, Offerhaus J, Maciejewski R, Polkowski W. Gastric cancer: epidemiology, prevention, classification, and treatment. Cancer Manag Res. 2018;10:239–48.
Rawla P, Barsouk A. Epidemiology of gastric cancer: global trends, risk factors and prevention. Prz Gastroenterol. 2019;14(1):26–38.
Cheng XJ, Lin JC, Tu SP. Etiology and prevention of gastric cancer. Gastrointest Tumors. 2016;3(1):25–36.
Forman D, Burley VJ. Gastric cancer: global pattern of the disease and an overview of environmental risk factors. Best Pract Res Clin Gastroenterol. 2006;20(4):633–49.
Dastmalchi, N., R. Safaralizadeh, and S.M. Banan Khojasteh, The correlation between microRNAs and Helicobacter pylori in gastric cancer. Pathog Dis, 2019. 77(4).
He RZ, Luo DX, Mo YY. Emerging roles of lncRNAs in the post-transcriptional regulation in cancer. Genes Dis. 2019;6(1):6–15.
Brosnan CA, Voinnet O. The long and the short of noncoding RNAs. Curr Opin Cell Biol. 2009;21(3):416–25.
Chen LL, Carmichael GG. Decoding the function of nuclear long non-coding RNAs. Curr Opin Cell Biol. 2010;22(3):357–64.
Hombach S, Kretz M. Non-coding RNAs: classification, biology and functioning. Adv Exp Med Biol. 2016;937:3–17.
Fernandes, J.C.R., et al., Long non-coding RNAs in the regulation of gene expression: physiology and disease. Noncoding RNA, 2019. 5(1).
Fu X, Tian Y, Kuang W, Wen S, Guo W. Long non-coding RNA DLX6-AS1 silencing inhibits malignant phenotypes of gastric cancer cells. Exp Ther Med. 2019;17(6):4715–22.
Sun, Q., et al., LncRNA LOXL1-AS1 facilitates the tumorigenesis and stemness of gastric carcinoma via regulation of miR-708-5p/USF1 pathway. Cell Prolif, 2019: p. e12687.
Liang Y, et al. DLX6-AS1/miR-204-5p/OCT1 positive feedback loop promotes tumor progression and epithelial-mesenchymal transition in gastric cancer. Gastric Cancer. 2019.
Tian X, Gao S, Liu Y, Xuan Y, Wu R, Zhang Z. Long non-coding RNA ENST00000500843 is downregulated and promotes chemoresistance to paclitaxel in lung adenocarcinoma. Oncol Lett. 2019;18(4):3716–22.
Bao Y, Tang J, Qian Y, Sun T, Chen H, Chen Z, et al. Long noncoding RNA BFAL1 mediates enterotoxigenic Bacteroides fragilis-related carcinogenesis in colorectal cancer via the RHEB/mTOR pathway. Cell Death Dis. 2019;10(9):675.
Zhang C, Liu J, Zhang Y, Luo C, Zhu T, Zhang R, et al. LINC01210 accelerates proliferation, invasion and migration in ovarian cancer through epigenetically downregulating KLF4. Biomed Pharmacother. 2019;119:109431.
Niland CN, Merry CR, Khalil AM. Emerging roles for long non-coding RNAs in cancer and neurological disorders. Front Genet. 2012;3:25.
Matsuoka T, Yashiro M. Biomarkers of gastric cancer: current topics and future perspective. World J Gastroenterol. 2018;24(26):2818–32.
Bolha L, Ravnik-Glavac M, Glavac D. Long noncoding RNAs as biomarkers in cancer. Dis Markers. 2017;2017:7243968.
Shi XY, et al. LncRNA CADM1-AS1 serves as a new prognostic biomarker for gastric cancer. Eur Rev Med Pharmacol Sci. 2019;23(3 Suppl):232–8.
Yang, J. and H. Song, Identification of long noncoding RNA RP11-169F17.1 and RP11-669N7.2 as novel prognostic biomarkers of stomach adenocarcinoma based on integrated bioinformatics analysis. Epigenomics, 2019.
Yang L, Long Y, Li C, Cao L, Gan H, Huang K, et al. Genome-wide analysis of long noncoding RNA profile in human gastric epithelial cell response to Helicobacter pylori. Jpn J Infect Dis. 2015;68(1):63–6.
Tao F, Tian X, Lu M, Zhang Z. A novel lncRNA, Lnc-OC1, promotes ovarian cancer cell proliferation and migration by sponging miR-34a and miR-34c. J Genet Genomics. 2018;45(3):137–45.
Kazemzadeh M, Safaralizadeh R, feizi MAHP, Somi MH, Shokoohi B. Misregulation of the dependence receptor DCC and its upstream lincRNA, LOC100287225, in colorectal cancer. Tumori. 2017;103(1):40–3.
Tam C, Wong JH, Tsui SKW, Zuo T, Chan TF, Ng TB. LncRNAs with miRNAs in regulation of gastric, liver, and colorectal cancers: updates in recent years. Appl Microbiol Biotechnol. 2019;103(12):4649–77.
Liu W, Xu J, Zhang C. Clinical usefulness of gastric adenocarcinoma predictive long intergenic noncoding RNA in human malignancies: a meta-analysis. Pathol Res Pract. 2019;215(6):152387.
Yang S, Sun Z, Zhou Q, Wang W, Wang G, Song J, et al. MicroRNAs, long noncoding RNAs, and circular RNAs: potential tumor biomarkers and targets for colorectal cancer. Cancer Manag Res. 2018;10:2249–57.
Liz J, Esteller M. lncRNAs and microRNAs with a role in cancer development. Biochim Biophys Acta. 2016;1859(1):169–76.
Slabakova E, et al. Alternative mechanisms of miR-34a regulation in cancer. Cell Death Dis. 2017;8(10):e3100.
Hermeking H. The miR-34 family in cancer and apoptosis. Cell Death Differ. 2010;17(2):193–9.
Rupaimoole R, Slack FJ. A role for miR-34 in colon cancer stem cell homeostasis. Stem Cell Investig. 2016;3:42.
Cheng CY, Hwang CI, Corney DC, Flesken-Nikitin A, Jiang L, Öner GM, et al. miR-34 cooperates with p53 in suppression of prostate cancer by joint regulation of stem cell compartment. Cell Rep. 2014;6(6):1000–7.
Jin K, Xiang Y, Tang J, Wu G, Li J, Xiao H, et al. miR-34 is associated with poor prognosis of patients with gallbladder cancer through regulating telomere length in tumor stem cells. Tumour Biol. 2014;35(2):1503–10.
Yang T, Zeng H, Chen W, Zheng R, Zhang Y, Li Z, et al. Helicobacter pylori infection, H19 and LINC00152 expression in serum and risk of gastric cancer in a Chinese population. Cancer Epidemiol. 2016;44:147–53.
Zhou X, Chen H, Zhu L, Hao B, Zhang W, Hua J, et al. Helicobacter pylori infection related long noncoding RNA (lncRNA) AF147447 inhibits gastric cancer proliferation and invasion by targeting MUC2 and up-regulating miR-34c. Oncotarget. 2016;7(50):82770–82.
The authors would like to express their deepest thanks to the staff of Imam Reza Hospital and Immunology Research Center of Tabriz University of Medical Sciences, Tabriz, Iran.
Conflict of Interest
The authors declare that they have no conflict of interest.
Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations.
About this article
Cite this article
Mohammadzadeh, A., Baradaran, B., Dastmalchi, N. et al. The Correlation Between Helicobacter pylori Infection and Lnc-OC1 Expression in Gastric Cancer Tissues in an Iranian Population. J Gastrointest Canc (2020). https://doi.org/10.1007/s12029-020-00438-4
- Gastric cancer
- Helicobacter pylori