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Prognostic and Diagnostic Values of miR-506 and SPON 1 in Colorectal Cancer with Clinicopathological Considerations

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Abstract

Purpose

One of the most common cancers in the world is colorectal cancer, which has increased significantly in recent decades. In the carcinogenicity process in the colon, there are genes involved in various cellular processes, such as cell cycle, apoptosis, and cell migration. According to studies carried out, both miR-506 and SPON 1 genes are involved in the process which initiates and promotes cancer. In this study, alterations in the expression of target genes from the viewpoint of carcinogenicity were studied and evaluated.

Methods

Fifty tumor tissues and normal marginal tissue were collected from patients who were undergoing colorectal cancer surgery. After the extraction of RNA, the real-time PCR method was performed to evaluate the gene expression. Also, alterations of gene expression in response to defined amounts of chemotherapeutic drugs (IC50) were evaluated. P < 0.05 was considered statistically significant.

Results

The relative expression level of miR-506 in tumor tissues was significantly decreased in comparison with healthy marginal tissues (P = 0.044). On the other hand, the SPON1 gene expression level was decreased too in tumor tissues in comparison with healthy marginal tissues (P = 0.019). There was also a significant relationship between the expression of target genes and clinicopathological involvement. However, there was no significant alteration in these genes along with the chemotherapeutic drug.

Conclusion

These findings suggest that the relative expression of miR-506 and SPON 1 gene can be considered as a diagnostic or predictive biomarker for colorectal cancer. However, further studies on protein levels should be conducted.

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Acknowledgments

This study was carried out and supported at the Immunology Research Center, Tabriz University of Medical Science, Tabriz, Iran.

Author information

Author notes

  1. Rozita Tamjidifar and Morteza Akbari contributed equally to this work.

Authors and Affiliations

  1. Immunology Research Center, Tabriz University of Medical Sciences, Tabriz, Iran

    Rozita Tamjidifar, Morteza Akbari, Saeed Tarzi & Navid Shomali

  2. Department of Biology Ahar Branch, Islamic Azad University, Ahar, Iran

    Rozita Tamjidifar & Saeed Tarzi

  3. Tuberculosis and Lung Disease Research Center, Tabriz University of Medical Sciences, Tabriz, Iran

    Mahsa Sadeghzadeh, Mahsa Abolghasemi & Elham Poursaei

  4. Student Research Committee, Tabriz University of Medical Sciences, Tabriz, Iran

    Navid Shomali

  5. Drug Applied Research Center, Tabriz University of Medical Sciences, Tabriz, Iran

    Farshad Mahdavi

  6. Department of General Surgery, Tabriz University of Medical Sciences, Tabriz, Iran

    Farshad Mahdavi

Authors
  1. Rozita Tamjidifar
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  2. Morteza Akbari
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  3. Saeed Tarzi
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  4. Mahsa Sadeghzadeh
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  5. Mahsa Abolghasemi
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  6. Elham Poursaei
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  7. Navid Shomali
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Correspondence to Farshad Mahdavi.

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Tamjidifar, R., Akbari, M., Tarzi, S. et al. Prognostic and Diagnostic Values of miR-506 and SPON 1 in Colorectal Cancer with Clinicopathological Considerations. J Gastrointest Canc 52, 125–129 (2021). https://doi.org/10.1007/s12029-019-00356-0

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  • DOI: https://doi.org/10.1007/s12029-019-00356-0

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