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Evaluation of Fecal M2PK as a Diagnostic Marker in Colorectal Cancer

  • Hisham K. Dabbous
  • Yosry Abd El-Rahman Mohamed
  • Runia F. El-Folly
  • Mohamed D. El-Talkawy
  • Hani E. Seddik
  • Dina Johar
  • Mohammed A. Sarhan
Original Research
  • 61 Downloads

Abstract

Background

Invasive colonoscopy is the gold standard for patients at risk for colorectal cancer. However, the need for non-invasive and specific markers is required.

Objective

To evaluate the sensitivity of the glycolytic pyruvate kinase isoenzyme type M2 dimer (M2PK) as a diagnostic biomarker for colorectal cancer (CRC) and adenomatous colorectal polyps (CRP) screening.

Design

Case-control.

Patients

Twenty patients with CRC, 20 patients with CRP (lack criteria for colonic cancer by biopsy), and 20 normal subjects.

Outcome

Complete blood count (CBC), erythrocyte sedimentation rate (ESR), tumor markers: carcino embryonic antigen (CEA) and carbohydrate antigen 19-9 (CA19-9), fecal occult blood test (FOBT), and fecal M2PK. Pelvic and abdominal ultrasound (US), colonoscopy, and a histopathological examination.

Results

Only weight loss and cachexia were significantly associated with CRC than CRP or control groups. M2PK was the most sensitive and specific test in differentiating CRC from CRP and the control subjects (sensitivity = 75%, specificity = 100%).

Limitations

(1) The selection of cases for three well-matched groups, as to perform colonoscopy in well-prepared cases and conditions. (2) Replicates in more than 20 cases for confirmation at the expense of enrolling new patients. (3) The cost associated with tumor markers analysis.

Conclusion

Fecal M2PK can be used as a precolonoscopy screening test for CRC patients, and is superior to other tumor markers, and in indicating the progress of colorectal adenomas > 1 cm. Thus being cost-effective and easy-to-perform test, it is a feasible tool to preselect patients who require colonoscopy.

Keywords

Fecal occult blood test Patients Colorectal cancer Colorectal polyp Marker Egypt 

Abbreviations

ANOVA

analysis of variance

BPR

bleeding per rectum

CA 19-9

carbohydrate antigen 19-9

CEA

carcino embryonic antigen

CRP

colorectal (adenomatous) polyps

CRC

colorectal cancer

CT

computerized tomography

Ctrl

control

DA

diagnostic accuracy

DM

diabetes mellitus

ELISA

enzyme-linked immunosorbent assay

ESR

erythrocyte sedimentation rate

FOBT

fecal occult blood test

HTN

hypertension

INR

international normalized ratio

LR

likelihood ratio

NCI

National Cancer Institute

NPV

negative predictive value

PTT

partial thromboplastin time

PT

prothrombin time

PPV

positive predictive value

M2PK

pyruvate kinase isoenzyme type M2

ROC

receiver operating characteristic

SPSS

Statistical Package for Social Sciences

AUC

area under the curve

Notes

Acknowledgements

All the authors contributed equally to conception, writing the manuscript, and the analysis of data included in this article. D.J. supported the Endnote X8 software required for this article.

Compliance with ethical standards

Conflict of interest

The authors declare that they have no conflict of interest.

Ethical approval

The study ethics protocol was approved by the Tropical Medicine Department, Ain-Shams University and the Gastroenterology and Hepatology Department, Theodor Bilharz Research Institute, Cairo, Egypt.

Consent to participate

Written consent to participate was given by all patients who were enrolled in the study.

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Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2018

Authors and Affiliations

  1. 1.Tropical Medicine Department, Faculty of MedicineAin Shams UniversityCairoEgypt
  2. 2.Hepatogastroenterology and Tropical Medicine DepartmentTheodor Bilharz Research InstituteCairoEgypt
  3. 3.Department of Biochemistry and Nutrition, Faculty of Women for Arts, Sciences and EducationAin Shams UniversityCairoEgypt
  4. 4.Department of Physiology and Pathophysiology, Max Rady College of Medicine, Rady Faculty of Health SciencesUniversity of ManitobaWinnipegCanada
  5. 5.Center of Excellence of Gastrointestinal Inflammation and Immunology Research (CEGIIR), Gastroenterology, Department of MedicineUniversity of AlbertaEdmontonCanada
  6. 6.National Liver Institute, Department of Medical Microbiology and ImmunologyMenofia UniversityShibin El-KomEgypt

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