Clinical Impact of p27 Kip1 and CaSR Expression on Primary Hyperparathyroidism
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We aimed to investigate the expressions of p27 kinase inhibitory protein 1 (p27 Kip1 ) and calcium sensing receptor (CaSR) in adenomas and normal parathyroid tissue and to evaluate the relationship of these molecules with clinical and biochemical parameters in primary hyperparathyroidism (PHPT). Fifty-one patients with histopathologically confirmed parathyroid adenomas and 20 patients with normal parathyroid glands (which were removed incidentally during thyroid resection) were included. Immunohistochemical stainings of CaSR and p27 Kip1 were performed in surgical specimens. Clinical features, biochemical parameters, and BMD measurements of patients with PHPT were evaluated retrospectively. Expressions of p27 Kip1 and CaSR were decreased in parathyroid adenomas, compared to normal glands (p < 0.05). High intensity of CaSR staining (3+) was more frequent in normal parathyroid tissue (75%) than adenomas (12%) (p < 0.01). Hypertension was not observed in patients with high staining intensity of CaSR (p = 0.032). There was a negative association between CaSR expression and body mass index (BMI) (p = 0.027, r = − 0.313). There was no significant relationship between p27 Kip1 and CaSR expressions, serum calcium, plasma parathormone, 25-hydroxy vitamin D levels, and bone density (p > 0.05). The expressions of p27 Kip1 and CaSR were decreased in PHPT patients. This reduction may play an important role in the pathogenesis of PHPT. However, neither p27 Kip1 nor CaSR expression was found to be useful in predicting prognosis or severity of disease.
KeywordsPrimary hyperparathyroidism Parathyroid adenoma p27Kip1 CaSR
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Conflict of Interest
The authors declare that they have no conflict of interest.
For this type of study, formal consent is not required.
The authors have nothing to disclose.
- 12.Assessment of fracture risk and its application to screening for postmenopausal osteoporosis. Report of a WHO Study Group. World Health Organ Tech Rep Ser. 1994;843:1–129.Google Scholar
- 16.Varshney S, Bhadada SK, Saikia UN, Sachdeva N, Behera A, Arya AK, et al. Simultaneous expression analysis of vitamin D receptor, calcium-sensing receptor, cyclin D1, and PTH in symptomatic primary hyperparathyroidism in Asian Indians. Eur J Endocrinol. 2013;169(1):109–116.CrossRefPubMedGoogle Scholar
- 23.Cifuentes M, Fuentes C, Mattar P, Tobar N, Hugo E, Ben-Jonathan N, et al. Obesity-associated proinflammatory cytokines increase calcium sensing receptor (CaSR) protein expression in primary human adipocytes and LS14 human adipose cell line. Arch Biochem Biophys. 2010;500(2):151–156.CrossRefPubMedGoogle Scholar