Establishment of a protocol to extend the lifespan of human hormone-secreting pituitary adenoma cells
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The aim of this study was to generate immortalized human anterior pituitary adenoma cells. Reliable cell models for the study of human pituitary adenomas are as yet lacking and studies performed so far used repeated passaging of freshly excised adenomas, with the attendant limitations due to limited survival in culture, early senescence, and poor reproducibility.
Methods & Results
We devised a technique based upon repeated co-transfections of two retroviral vectors, one carrying the catalytic subunit of human telomerase, hTERT, the other SV40 large T antigen. This approach extended the lifespan of cells derived from a human growth hormone-secreting adenoma up to 18 months while retaining morphology of primary cells, growth hormone synthesis and growth hormone secretion.
Our attempt represents the first demonstration of successful lifespan extension of human growth hormone-secreting pituitary adenoma cells via co-transfection of hTERT and SV40T and paves the way to future attempts to obtain stable cell lines.
KeywordsGH-secreting adenoma Immortalization Pituitary cell line TERT SV40 T-antigen
This work was supported by the Italian Ministry of Education, University and Research (PRIN2010TYCL9B_006) to AF, (PRIN2010TYCL9B_004) to FPG, (PRIN2010TYCL9B-001) and PRIN 2015 (PRIN2015ZHKFTA) to SC; and by the Italian Ministry of Health (GR 2011-02351557) to SN and (RF-2013-02356201) to SC.
Compliance with ethical standards
Conflict of interest
The authors declare that they have no competing interests.
All procedures performed in studies involving human participants were in accordance with the ethical standards of the institutional and/or national research committee and with the 1964 Helsinki declaration and its later amendments or comparable ethical standards.
Informed consent was obtained from all individual participants included in the study.