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Fig. 3 | Endocrine

Fig. 3

From: Leptin stimulation of cell cycle and inhibition of apoptosis gene and protein expression in OVCAR-3 ovarian cancer cells

Fig. 3

Effects of leptin (40 ng/ml) on a cyclin D1 (36 kDa), b cyclin A (55 kDa), and c p21WAF1/CIP1 (21 kDa) protein expression in OVCAR-3 cells. Cells were treated with leptin for 24 and 48 h. β-actin (42 kDa) served as the loading control, and densitometry values of cyclin D1, cyclin A, and p21WAF1/CIP1 bands were normalized to β-actin. Signal intensities are expressed in arbitrary units. The values are mean ± SEM. **,***Significantly different from control at P < 0.01 and P < 0.001, respectively

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