Abstract
The early stage of inflammation involves the adhesion and transmigration of leukocytes across the blood–brain barrier (BBB) to the normally sequestered central nervous system (CNS). This process is regulated by the expression of a series of adhesion molecules. One of the most well-known components is intercellular adhesion molecule-1 (ICAM-1). It was described as a ligand of the membrane-bound integrin receptors lymphocyte function-associated antigen-1 (LFA-1) and monocyte adhesion molecules-1 (Mac-1) on leukocytes, and was involved in the adhesion and transmigration of leukocytes. Studies have demonstrated the upregulation of ICAM-1 in many tissues after lipopolysaccharide (LPS) stimulation, for example. In the CNS, recent studies just focus on the relatively acute effects in brain tissues, but neglected the possibly existed differences between the brain and the spinal cord following traumatic lesions. Our data demonstrated the upregulation of ICAM-1, LFA-1, and Mac-1 in the spinal cords of LPS intraspinal injected rats, and the location of ICAM-1 in microglia cells. These results suggested a possible role of this molecule in microglia-mediated immune response and antigen presenting in CNS immune diseases.
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Abbreviations
- ICAM-1:
-
Intercellular adhesion molecule-1
- MS:
-
Multiple sclerosis
- LPS:
-
Lipopolysaccharid
- LFA-1:
-
Lymphocyte function-associated antigen-1
- Mac-1:
-
Monocyte adhesion molecules-1
- BBB:
-
Blood–brain barrier
- TNF:
-
Tumor necrosis factor
- CNS:
-
Central nervous system
- GAPDH:
-
Glyceraldehyde-3-phosphate dehydrogenase
- PAGE:
-
Polyacrylamide gel electrophoresis
- ECL:
-
Enhanced chemiluminescence system
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Acknowledgments
This work was supported by the National Natural Scientific Foundation of China Grant (No. 30300099 and No. 30770488), Natural Scientific Foundation of Jiangsu Province Grant (No. BK2003035 and No. BK2006547), College and University Natural Scientific Research Programme of Jiangsu Province (No. 03KJB180109 and No. 04KJB320114), Technology Guidance Plan for Social Development of Jiangsu Province Grant (BS2004526), Health Project of Jiangsu Province (H200632), The foundation of Talented Person at the Summit of 6 Fields of Jiang Su Province. (No. 2).
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The authors Junling Yang and Min Fei have contributed equally to this article.
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Yang, J., Fei, M., Gu, Y. et al. Evaled Expression of ICAM-1 and Its Ligands in the Rat Spinal Cord Following Lipopolysaccharide Intraspinal Injection. Neuromol Med 10, 385–392 (2008). https://doi.org/10.1007/s12017-008-8049-7
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DOI: https://doi.org/10.1007/s12017-008-8049-7