C3G is essential for the regulation of cell adhesion. a Heat map showing differential expression of cell adhesion-related genes in C3G KO cells compared to WT cells. b Western blots showing levels of indicated proteins. Actin was used as a loading control. Relative levels averaged from three experiments are shown in the bar diagram. pFAK and pPaxillin were normalized against corresponding total proteins.***p < 0.001, n = 3. c C3G knock out cells show reduced adhesion, Quantification of adherent colonies at indicated time points upon trypsin treatment is shown in bar diagram (see also S4C), ***p < 0.001, n = 3. d Western blots showing E-cadherin protein levels in the indicated cells. Actin was used as loading control and quantitation of the expression averaged from multiple experiments is shown in the bar diagram p < 0.001, n = 4.