ESCs lacking C3G fail to differentiate in vitro and in vivo. a Representative images (bright-field) of 12 days old EBs formed by WT and C3G null clone (D1). Arrowhead indicates the cystic cavity. Scale bar, 200 μm. b qPCR analysis of C3G expression during EB differentiation in WT cells c qPCR analysis of expression of the indicated genes during EB differentiation, RNA samples were collected from Day 0 to Day 12, and GAPDH was used as an internal control. n = 3. d H&E stained histological sections of 21-day old teratomas. Images show representative sections taken from different areas of the teratoma. A-Adipocytes; C-Cartilage; G-Gut Endoderm; NE-neuroepithelium. Scale bar, 100 μm.